实验性分级大鼠脊髓损伤模型的建立

Establishment of a new experimental rat model with grading spinal cord injury

目的分级建立大鼠不同程度脊髓损伤模型。方法 140只大鼠均分为四组,采用自行研制的一种新型电控大鼠脊髓损伤打击器,以恒定的重物和打击时间,观察打击高度为1.5、2.5和6cm造成脊髓损伤后的后肢运动功能、电生理和病理变化。结果假手术组苏醒后能站立行走,CSEP-P波潜伏期恒定,脊髓结构正常。打击高度为1.5cm时(B组),动物出现轻度瘫换,28d后能站立行走,CSEP-P波延长,脊髓结构破坏较轻;打击高度为2.5cm时(C组),动物出现不全瘫,28d后不能站立行走,双后肢不能支持负重,CSEP-P波显著延长,脊髓结构破坏明显;打击高度为6.0cm时(D组),动物出现全瘫,28d后双后肢无运动,CSEP-P波始终呈直线,脊髓结构破坏严重。结论自行研制的脊髓损伤打击仪能够实现定时、定点、定高打击,可以分级、稳定的复制出轻、中、重度的脊髓损伤模型。

Objective To establish a new rat model with grading spinal cord injury.MethodsUsing an electronic spinal cord injury-making device made ourselves,140 adult rats were equally devided into 4 groups.The rats in group S were taken as sham operated.The rats in groups of B,C and D were beaten in different beating heights of 1.5 cm,2.5 cm and 6.0 cm,respectively,under constant weight and beating time.The motor function,electrophysiology and pathological changes were observed.Results The rats of group A could walk after recovered from anesthesia,the latency of CSEP-P wave was 17-18 ms,and structure of the spinal cord was normal.The rats in group B showed a slightly prolonged CSEP-P wave and injuried spinal cord pathologically,which were mildly paralyzed and could walk 28 days later.The rats in group C showed a significantly prolonged CSEP-P wave and moderately injuried spinal cord pathologically,which were partially paralyzed and could not walk 28 days later.The rats in group D showed a lineal CSEP-P wave all the time and severe pathological spinal cord injury,which were paralyzed completely and had no any movement four weeks of injury.Conclusion The rat model with grading spinal cord injury can be established using the electronic spinal cord injury-making device by different beating heights under constant weight and beating time.