胞内氯离子通道蛋白1表达与胆囊癌侵袭转移的相关性研究

CLIC1 protein expression correlates with invasion and metastasis of gallbladder cancer

目的探讨胆囊癌组织胞内氯离子通道蛋白1（chloride intraeellular channel 1，CLICl）蛋白表达与胆囊癌侵袭转移的关系。方法应用免疫组织化学sP染色法及Westernblot技术，检测36例胆囊癌和相应癌旁组织中CLICl蛋白的表达。采用Spearman等级相关分析、x。检验对结果进行分析。结果免疫组化结果提示，CLICl蛋白高表达于胆囊癌组织，癌旁组织中CLICl蛋白表达较少，阳性表达率分别为81％（29／36）和44％（16／36），差异有统计学意义（P〈0．05）。胆囊癌组织CLICl表达与患者性别和年龄无关（P〉0．05），而与肿瘤的淋巴转移、浸润深度、TNM分期和分化程度相关（P〈0．05）。Westernblot结果显示，CLICl的特异性条带出现在Marker标准相对分子质量27000左右，CLICl在胆囊癌的阳性率高于癌旁组织（P〈0．05）。结论CLICl可能在胆囊癌的发生发展过程中发挥重要的作用，CLICl蛋白的高表达与胆囊癌的侵袭转移密切相关。

Objective To investigate the correlation between the CLIC1 protein expression with the invasion and metastasis in gallbladder cancer. Methods The expressions of CLIC1 was detected in 36 matching-samples including primary gallbladder cancer tissues and paratumor normal tissues by immunohistochemistry and Western blot. All data were analyzed via Spearman rank correlation coefficient and chi-square test. Results The over-expression of CLIC1 was found in 81% （29/36）of the gallbladder cancer tissues, whereas in 44% （16/36） of paratumor normal tissues. There were significant differences in the expression of CLIC1 between them （P 〈0. 05）. The expression of CLIC1 did not correlate with age and sex of patients（P 〉 0. 05 ）, but with tumor differentiation and lymph node metastasis （P 〈 0.05 ）. Western blot displayed clear bands of CLIC1 about 27 000 by molecular weight marker Mix, and there was a significant difference between the high expressions of CLIC1 in gallbladder cancer and the low expressions in paratumor normal tissues（P 〈 0.05）. Conclusions CLIC1 may play an important role in tumorigenesis of gallbladder cancer. The invasion and metastasis of gallbladder cancer lesions are correlated with the expression of CLIC1.