期刊文献+

乌司他丁逆转大鼠肝纤维化的实验研究 被引量:1

Ulinastai reverses experimental hepatofibrosis in rats
原文传递
导出
摘要 目的探讨乌司他丁对大鼠肝纤维化过程的影响。方法18只TAA诱导的肝纤维化模型大鼠分为3组:正常大鼠为A组(3只),模型鼠+生理盐水为B组(9只),模型鼠+乌司他丁为c组(9只)并干预3周。对比干预前后大鼠血清中的丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)、超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、透明质酸(hyaluronic acid、HA)、层粘连蛋白(1aminin,LN)水平变化。病理学检查及免疫组化观察各组大鼠肝形态学变化及转化生长因子β1(transforminggrowthfactorB1,TGF.t31)、caspase-3蛋白表达。结果c组干预后大鼠肝脏弹性数值及SSS评分与干预前相比显著降低,差异有统计学意义(t=2.472,P〈0.05)。c组的ALT、AST、SOD、MDA、HA、LN生化指标均有不同程度的好转(分别F=3.862、5.774、3.442、4.157、4.173、3.674,均P〈0.05)。HE及Masson染色可见干预后C组大鼠肝组织有少量炎性细胞浸润,肝细胞坏死灶并未增多,胶原纤维略有增生。C组TGF-β1、caspase-3阳性细胞率明显低于B组大鼠。结论乌司他丁对大鼠肝纤维化具有逆转作用,使大鼠肝脏纤维化程度逐渐减轻,减少胶原纤维的沉积,抑制肝细胞炎症。 Objective To explore if Ulinastai reverses hepatofibrosis in rats. Method Rat hepatofibrosis models were induced by TAA subcutaneously injection. 18 rats with hepatofibrosis were divided into 2 groups, Ulinastai treatment group (9 rats), normal saline control group (9 rats). AST, ALT, HA, SOD, MDA, LN level were measured and compared between the 2 groups and that of healthy rats (3 rats). Rats liver morphology was observed using HE, Masson stain and type-B ultrasonic. TGF-β1, caspase-3 were detected by immunohistochemistry. Result After 3 weeks Ulinastai treatment, elastic index and SSS score in Ulinastai group decreased significantly compared to pretreatment ( t = 2. 472, P 〈 0. 05). ALT, AST, SOD, MDA, HA and LN level significantly improved (F = 3. 862, 5. 774, 3.442, 4. 157, 4. 173, 3. 674, P 〈 0. 05). Compared with NS treatment group HE and Masson staining showed fewer inflammatory ceils infiltration in central vein, ballooning degeneration of liver cells, collagen proliferated, hepatic lobules degradation and pseudolobule arise after 3 weeks intervenion. Also in Ulinastai group, TGF-β1, caspase-3 positive cells were much less than that in NS treatment group. Conclusions Ulinastai can reverse rat hepatic fibrosis and alleviate fibrosis degree and collagen fiber
出处 《中华普通外科杂志》 CSCD 北大核心 2013年第2期138-141,共4页 Chinese Journal of General Surgery
基金 广东省自然科学基金资助项目(10151006001000013)
关键词 肝硬化 转化生长因子Β1 半胱氨酸天冬氨酸蛋白酶3 乌司他丁 Liver cirrhosis Transforming growth factor betal Caspase-3 Ulinastai
  • 引文网络
  • 相关文献

参考文献10

  • 1Kanta J,Dooley S,Delvoux B. Tropoelastin expression is up-regulated during activation of hepatic stellate cells and in the livers of CCl4-cirrhotic rats[J].Liver,2002.220-227.
  • 2Fang HL,Lin WC. Corn oil enhancing hepatic lipid peroxidation induced by CC14 does not aggravate liver fibrosis in rats[J].Food and Chemical Toxicology,2008.2267-2273.
  • 3Shaker ME,Salem HA,Shiha GE. Nilotinib counteracts thioacetamide-induced hepatic oxidative stress and attenuates liver fibrosis progression[J].Fundamental and Clinical Pharmacology,2011.248-257.
  • 4Ding S,Schultz PG. A role for chemistry in stem cell biology[J].Nature Biotechnology,2004.833-840.
  • 5Morrison RF,Farmer SR. Hormonal signaling and transcriptional control of adipocyte differentiation[J].Journal of Nutrition,2000.3116S-3121S.
  • 6Wong F,Liu P,Blendis L. The mechanism of improved sodium homeostasis of low-dose losartan in preascitic cirrhosis[J].Hepatology,2002.1449-1458.
  • 7Tuch BE. Stem cells:a clinical update[J].Australian Family Physician,2006.719-721.
  • 8Song YH,Chen XL,Kong XJ. Ribozymes against TGFβ1reverse character of activated hepatic stellate cells in vitro and inhibit liver fibrosis in rats[J].Journal of Gene Medicine,2005.965-976.
  • 9张广林,罗蒙,孙勇伟,徐庆,陈炜.小RNA干扰DDR2基因表达对肝星状细胞的影响[J].中华普通外科杂志,2009,24(9):748-751. 被引量:3
  • 10刘峰,刘志达,武楠,丛旭,费然,陈红松,魏来.内皮祖细胞移植对大鼠肝硬化作用的研究[J].中华普通外科杂志,2009,24(1):53-56. 被引量:3

二级参考文献17

  • 1梅雀林,刘鹏程,李彦豪.血管内皮祖细胞在血管生物学中的作用[J].中华医学杂志,2005,85(25):1793-1796. 被引量:5
  • 2Rafii S, Lyden D. Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med, 2003, 9:702-712.
  • 3Jia L, Takahashi M, Yoshioka T, et al. Therapeutic potential of endothelial progenitor ceils for cardiovascular diseases. Curr Vasc Pharmacol, 2006,4:59- 65.
  • 4Liew A, Barry F, O' Brien T. Endothelial progenitor cells: diagnostic and therapeutic considerations. Bioessays, 2006, 28 : 261-270.
  • 5Ishak K, Baptista A, Bianchi L, et al. Histological grading and staging of chronic hepatitis, J Hepatol, 1995, 22:696-699.
  • 6Medina J, Arroyo AG, Sanchez-Madrid F, et al. Angiogenesis in chronic inflammatory liver disease. Hepatology, 2004, 39 : 1185-1195.
  • 7Corpechot C, Barbu V, Wendum D, et al. Hypoxia-induced VEGF and collagen Ⅰ expressions are associated with angiogenesis and fibrogenesis in experimental cirrhosis. Hepatology, 2002, 35 : 1010-1021.
  • 8Taniguchi E, Kin M, Torimura T, et al. Endothelial progenitor cell transplantation improves the survival following liver injury in mice. Gastroenterology, 2006, 130: 521-531.
  • 9Bataller B,Brenner DA.Liver fibrosis.J Clin Invest,2005,115:209-218.
  • 10Vogel W.Discoidin domain receptom:structural relations and functional implications.FASEB J,1999,13:S77-S82.

共引文献4

同被引文献9

引证文献1

二级引证文献9

;
使用帮助 返回顶部