摘要
目的探讨乌司他丁对大鼠肝纤维化过程的影响。方法18只TAA诱导的肝纤维化模型大鼠分为3组:正常大鼠为A组(3只),模型鼠+生理盐水为B组(9只),模型鼠+乌司他丁为c组(9只)并干预3周。对比干预前后大鼠血清中的丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)、超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、透明质酸(hyaluronic acid、HA)、层粘连蛋白(1aminin,LN)水平变化。病理学检查及免疫组化观察各组大鼠肝形态学变化及转化生长因子β1(transforminggrowthfactorB1,TGF.t31)、caspase-3蛋白表达。结果c组干预后大鼠肝脏弹性数值及SSS评分与干预前相比显著降低,差异有统计学意义(t=2.472,P〈0.05)。c组的ALT、AST、SOD、MDA、HA、LN生化指标均有不同程度的好转(分别F=3.862、5.774、3.442、4.157、4.173、3.674,均P〈0.05)。HE及Masson染色可见干预后C组大鼠肝组织有少量炎性细胞浸润,肝细胞坏死灶并未增多,胶原纤维略有增生。C组TGF-β1、caspase-3阳性细胞率明显低于B组大鼠。结论乌司他丁对大鼠肝纤维化具有逆转作用,使大鼠肝脏纤维化程度逐渐减轻,减少胶原纤维的沉积,抑制肝细胞炎症。
Objective To explore if Ulinastai reverses hepatofibrosis in rats. Method Rat hepatofibrosis models were induced by TAA subcutaneously injection. 18 rats with hepatofibrosis were divided into 2 groups, Ulinastai treatment group (9 rats), normal saline control group (9 rats). AST, ALT, HA, SOD, MDA, LN level were measured and compared between the 2 groups and that of healthy rats (3 rats). Rats liver morphology was observed using HE, Masson stain and type-B ultrasonic. TGF-β1, caspase-3 were detected by immunohistochemistry. Result After 3 weeks Ulinastai treatment, elastic index and SSS score in Ulinastai group decreased significantly compared to pretreatment ( t = 2. 472, P 〈 0. 05). ALT, AST, SOD, MDA, HA and LN level significantly improved (F = 3. 862, 5. 774, 3.442, 4. 157, 4. 173, 3. 674, P 〈 0. 05). Compared with NS treatment group HE and Masson staining showed fewer inflammatory ceils infiltration in central vein, ballooning degeneration of liver cells, collagen proliferated, hepatic lobules degradation and pseudolobule arise after 3 weeks intervenion. Also in Ulinastai group, TGF-β1, caspase-3 positive cells were much less than that in NS treatment group. Conclusions Ulinastai can reverse rat hepatic fibrosis and alleviate fibrosis degree and collagen fiber
出处
《中华普通外科杂志》
CSCD
北大核心
2013年第2期138-141,共4页
Chinese Journal of General Surgery
基金
广东省自然科学基金资助项目(10151006001000013)