摘要
目的探讨原发性肝癌中白细胞介素-17A(IL-17A)的表达水平及其与肿瘤生长的相关性。方法采用RT-PCR法和免疫组织化学法分别检测人原发性肝癌组织中IL-17AmRNA和CD34分子的表达水平。体外培养小鼠肝癌细胞株H22,加入不同浓度的IL-17A(0.1、0.5、1.0、5.0、10、50、100、500、1000ng/m1),MTT法检测H22细胞的增殖情况。建立小鼠的H22细胞株肝癌模型,尾静脉输注外源性IL-17A,观察肿瘤生长情况并检测肿瘤组织中CD31分子的表达。结果37例原发性肝癌标本中,IL-17AmRNA阳性的有26例。CD34分子染色显示IL-17AmRNA(-4-)组的表达明显高于IL-17AmRNA(—)组,计数肿瘤微血管密度分别为66.6±2.5和26.7±2.5(P〈O.01)。体外培养H22细胞,加入不同浓度的IL一17A后,细胞增殖实验组与对照组差异无统计学意义(P〉O.05)。尾静脉输注IL一17A组小鼠肝癌肿瘤体积(843.6土90.9mm。)明显大于输注生理盐水组(198.7±24.4mm。),差异有统计学意义(P〈O.01)。CD31分子染色输注IL-17A组高于对照组,计数微血管密度分别为71.9±6.8和33.3±2.9(P〈O.01)。结论大多数人原发性肝癌组织中均有IL-17AmRNA表达。外源性IL-17A对小鼠H22肝癌细胞株的体外生长没有直接影响。尾静脉输注IL-17A后,H22肝癌细胞株在小鼠体内生长明显加快,同时有高密度的微血管生成,说明IL-17A可促进肝癌肿瘤生长,促进肿瘤微血管生成可能是其机制之一。
Objective To study the expression of interleukin (IL-17A) in primary liver cancer (PHC) tissue and its clinical significance. Methods Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry was performed respectively to detect the expression of IL-17A mRNA and CD34 in tumor tissues from 37 patients with PHC. Murine H22 cells cultured in vitro were exposed to IL-17A at different dosages (0. 1, 0.5, 1.0, 5.0, 10, 50,100, 500, 1000 ng/ml) and the cell proliferation was assayed by MTT. IL-17A was administered to the mice transplanted with H22 cancer cells via caudal vein and the tumor volume was measured by vernier caliper. Immunohisto- chemistry was used to detect the CD31 expression in H22 cancer tissues. Results IL-17A mRNA was detected in 26 of the 37 samples of primary liver cancer. The microvessel densities in IL-17A-positive samples and IL-17A-negative samples were 66.6 ~ 2.5 and 26.7 土2.5, respectively. The difference between two groups was significant (P〈0.01). The proliferation of H22 cells exposed to various dosages of IL-17A was not different (P〉0.05). The volume of murine tumor tissue in animals treated with IL-17A was (843.6土 90.9) mm3 and in untreated animals was (198.74-24.4) mm3 (P〈0.01). The microvessel density in treated group and control group was 71.9土6.8 and 33.3土2.9, respectively (P〈0.01). Conclusions The expression of IL-17A could be detected in a considerable proportion of primary liver cancers and correlated with angiogenesis. Exogenous IL-17A could not accelerate H22 cell proliferation in vitro but in vivo probably via enhancing angiogenesis.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2013年第2期143-146,共4页
Chinese Journal of Hepatobiliary Surgery