原发性肝癌中白细胞介素-17A的表达及其临床意义

Expression of interleukin-17A in primary liver cancer and its clinical significance

目的探讨原发性肝癌中白细胞介素-17A（IL-17A）的表达水平及其与肿瘤生长的相关性。方法采用RT-PCR法和免疫组织化学法分别检测人原发性肝癌组织中IL-17AmRNA和CD34分子的表达水平。体外培养小鼠肝癌细胞株H22，加入不同浓度的IL-17A（0．1、0．5、1．0、5．0、10、50、100、500、1000ng／m1），MTT法检测H22细胞的增殖情况。建立小鼠的H22细胞株肝癌模型，尾静脉输注外源性IL-17A，观察肿瘤生长情况并检测肿瘤组织中CD31分子的表达。结果37例原发性肝癌标本中，IL-17AmRNA阳性的有26例。CD34分子染色显示IL-17AmRNA（-4-）组的表达明显高于IL-17AmRNA（—）组，计数肿瘤微血管密度分别为66．6±2．5和26．7±2．5（P〈O．01）。体外培养H22细胞，加入不同浓度的IL一17A后，细胞增殖实验组与对照组差异无统计学意义（P〉O．05）。尾静脉输注IL一17A组小鼠肝癌肿瘤体积（843．6土90．9mm。）明显大于输注生理盐水组（198．7±24．4mm。），差异有统计学意义（P〈O．01）。CD31分子染色输注IL-17A组高于对照组，计数微血管密度分别为71．9±6．8和33．3±2．9（P〈O．01）。结论大多数人原发性肝癌组织中均有IL-17AmRNA表达。外源性IL-17A对小鼠H22肝癌细胞株的体外生长没有直接影响。尾静脉输注IL-17A后，H22肝癌细胞株在小鼠体内生长明显加快，同时有高密度的微血管生成，说明IL-17A可促进肝癌肿瘤生长，促进肿瘤微血管生成可能是其机制之一。

Objective To study the expression of interleukin （IL-17A） in primary liver cancer （PHC） tissue and its clinical significance. Methods Reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry was performed respectively to detect the expression of IL-17A mRNA and CD34 in tumor tissues from 37 patients with PHC. Murine H22 cells cultured in vitro were exposed to IL-17A at different dosages （0. 1, 0.5, 1.0, 5.0, 10, 50,100, 500, 1000 ng/ml） and the cell proliferation was assayed by MTT. IL-17A was administered to the mice transplanted with H22 cancer cells via caudal vein and the tumor volume was measured by vernier caliper. Immunohisto- chemistry was used to detect the CD31 expression in H22 cancer tissues. Results IL-17A mRNA was detected in 26 of the 37 samples of primary liver cancer. The microvessel densities in IL-17A-positive samples and IL-17A-negative samples were 66.6 ~ 2.5 and 26.7 土2.5, respectively. The difference between two groups was significant （P〈0.01）. The proliferation of H22 cells exposed to various dosages of IL-17A was not different （P〉0.05）. The volume of murine tumor tissue in animals treated with IL-17A was （843.6土 90.9） mm3 and in untreated animals was （198.74-24.4） mm3 （P〈0.01）. The microvessel density in treated group and control group was 71.9土6.8 and 33.3土2.9, respectively （P〈0.01）. Conclusions The expression of IL-17A could be detected in a considerable proportion of primary liver cancers and correlated with angiogenesis. Exogenous IL-17A could not accelerate H22 cell proliferation in vitro but in vivo probably via enhancing angiogenesis.