RPMI-1640和DMEM/F12培养基对大鼠颈环上皮细胞培养的比较研究

Comparative study of the cervical-loop epithelia cultured in RPMI-1640 and DMEM/F12 two different media

目的:比较大鼠颈环上皮细胞在不同培养基中的生长状况。方法:分离培养大鼠颈环上皮细胞,分别采用RPMI-1640和DMEM/F12两种培养基培养,用倒置显微镜观察细胞的生长状态,MTT比色法检测细胞增殖活性。结果:倒置显微镜下,两组细胞在接种24 h后细胞大量贴壁并伸展,14 d时RPMI-1640组细胞呈现明显的凋亡现象,DMEM/F12组细胞生长状态良好。两种方法培养的颈环上皮细胞增殖活性差异有显著性意义(P﹤0.05)。结论:DMEM/F12培养基较RPMI-1640更适合颈环上皮细胞的贴壁及增殖。

Objective： To compare the growth condition of the cervical-loop epithelia under different culture conditions, and to search for a simple and practical in vitro culture method of the cervical-loop epithelia. Method： Isolation and culture the cervical-loop epithelial cells in the control group were cultured in RPMI-1640 medium, cells in the experimental group were cultured in DMEM/F12 medium, the medium contained the same component as control group under the same condition. Cell adherence, morpholbgy and proliferation were observed under an inverted microscope. Cell proliferation activity was detected by MTF. Result： Under the inverted microscope, cells were adhered and expanded 24 hours after incubation in both groups. At 14 days,control group cells were apoptosis, experiment group cells were growed well.Significant difference was detected in the cervical-loop epithelia proliferation by both methods （P 〈 0.05）. Conclusion： DMEM / F12 medium than in RPMI-1640 is more suitable for the attachment and proliferation of the cervical loop epithelial ceils.