摘要
目的观察胚胎植入期二硫化碳(Cs2)暴露对孕鼠血清雌二醇(E2)含量和子宫组织雌激素受体d(ER-α)表达水平的影响,探讨Cs2致胚胎毒性的作用机制。方法健康雌性小鼠分别在受孕后第3天(GD3)、第4天(GD4)、第5天(GD5)、第6天(GD6)暴露于单一剂量(631.4mg/kg)的CS2或橄榄油(溶剂对照),单次暴露之后在不同的观察终点结束实验。采用酶联免疫吸附试验(ELISA)检测各观察终点孕鼠血清E2含量,采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS)和蛋白免疫印迹法(Western blotting)检测各观察终点子宫组织ER-α表达水平。结果GD3、GD4、GD5和GD6各暴露组在孕后第7天观察终点血清E2水平有不同程度的下降,差异均有统计学意义(P〈0.05);GD3和GD5暴露组孕鼠在孕后第6天观察终点其血清E2水平也有所下降,差异有统计学意义(P〈0.05)。Western blotting结果显示,GD4和GD5暴露组孕鼠子宫组织中ER-α均在暴露后第一个观察终点(即孕后第5天和孕后第6天)低表达,表达水平分别较对照组降低了23.6%和72.9%,差异均具有统计学意义(P〈0.05);GD3和GD6暴露组孕鼠在各观察终点ER-α表达水平均较对照组有所降低,但差异无统计学意义(P〉0.05)。结论胚胎植入期Cs2暴露可降低子宫组织ER-α表达水平并减少孕鼠血清E2含量,这可能是Cs2致胚胎毒性的分子机制之一。
Objective Toevaluate the effects ofcarbon disulfide (CS2) exposure during peri-implantation on the estrogen receptor-α (ER-α) expression in the uterus and serum level of estradiol (E2) in pregnant mice, and to explore the mechanism of embryotoxicity of CS2. Methods Healthy female mice were exposed to a single dose of CS= (631.4 mg/kg) or olive oil (solvent control) on gestational day (GD)3, GD4, GD5, or GD6. At different time points after exposure, the serum E2 levels of the pregnant mice were measured by enzyme- linked immunosorbent assay, and the expression levels of ER-α in the uterus were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot. Results Compared with the control group, the GD3, GD4, GD5, and GD6 exposure groups showed significantly decreased serum E2 levels on day 7 of gestation (P〈0.05); the GD3 and GD5 exposure groups showed significantly decreased serum E2 levels on day 6 of gestation (P〈0.05). The expression level of ER-α in the GD 4 exposure group was 23.6% lower than that in the control group on day 5 of gestation, and the expression level of ER-α in the GD 5 exposure group was 72.9% lower than that in the control group on day 6 of gestation (P〈0.05); the GD 3 and GD 6 exposure groups showed lower expression levels of ER-α than the control group at any time point, but no significant difference was found (P〉0.05). Conclusion CS2 exposure during peri-implantation can reduce the ER-α expression in the uterus and the serum level of E2 in pregnant mice, which may be one of the mechanisms of embryotoxicity of CS2.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2013年第2期88-91,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金面上项目(81072275)
关键词
二硫化碳
胚胎植入
雌激素
雌激素受体Α
Carbon disulfide
Peri-implantation
Estrogen
Estrogen receptor-α