两纯系鸡胃肠道黏膜细菌l6S rDNA的PCR-DGGE分析比较

Comparative Analysis of 16S rDNA of the Gastrointestinal Mucosa-associated Microbiota between Two Pure Lines Chicken by PCR-DGGE

以1日龄纯系的优质肉鸡A系(♂,A组)和惠阳胡须鸡(♂,B组)为实验动物,研究比较两纯系鸡1～28日龄胃肠道黏膜细菌微生物丛组成与动态变化特点。采用l6SrDNAV3PCR-DGGE的方法,分析第1、3、7、14、21、28日龄2组鸡胃肠道黏膜细菌基因组DNA。DGGE图谱中显示,28日龄2组鸡回肠泳道的条带数差异明显,嗉囊、腺胃及盲肠黏膜菌群结构的相似性均大于70%,十二指肠与空肠的相似性分别为40%和44%,回肠仅18%;21～28日龄2组鸡回肠黏膜菌群结构发生变化的程度不同,A组的动态变化值(%change)为70%,而B组仅11%。优质肉鸡A系和惠阳胡须鸡28日龄回肠黏膜菌群结构存在明显差异,这种差异可能是宿主遗传的影响所致,同时也是影响肉鸡生产性能的可能原因。

The experiment were conducted to analyze and compare the diversity and dynamic change of the gastrointestinal mucosa-associated microbiota（MAM） of high quality chicken line A（Pure Line♂,Group A） and Huiyang Beard chicken（Pure Line♂,Group B） during day 1-28.The V3 region of 16S rDNA of the mucosa-associated microbiota of two chicken pure lines were analyzed by Polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） at day 1,3,7,14,21 and 28,respectively.DGGE profiles,based on the number of bands of the gel,indicated the MAM of ileum between the two groups were significantly different at day 28.The similarity of the MAM in craw,glandular stomach and cecum between the two groups were higher than 70%,while the similarity in duodenum,jejunum and ileum were 40%,44% and 18% at day 28,respectively.The alterations of MAM of ileum were remarkably different between the two groups during day 21-28,with 70% change in group A and 11% change in group B.This diversity between the high quality chicken line A and Huiyang Beard chicken might be influenced by the host genetic and,at the same time,might be one of the possible cause for their different growth performance.