孕酮、干扰素τ对牛子宫内膜上细胞外基质及其相关配体表达的影响

Effects of Progesterone,Interferon-τ on Expression of the Related Genes of Extracellular Matrix-Their Ligands in Cultured Bovine Endometrial Cells

为探索干扰素τ(IFN-τ)、孕酮对牛子宫内膜上细胞外基质(ECM)及其配基基因表达的影响,本研究在体外培养的牛子宫内膜细胞(bECs)培养液中,分别添加孕酮、bIFN-τ、孕酮和bIFN-τ混合物培养24h后,通过荧光定量PCR技术,检测bECs上Collagen、Laminin、Fibronectin、THBS和Tenascin等ECM基因和ECM配基基因Syndecan、CD44、CD47和RHAMM的表达。结果表明:在体外培养的牛子宫内膜细胞上,bIFN-τ显著地诱导了Tenascin、Collagen、RHAMM和Laminin基因的表达,孕酮显著地诱导了CD44、Fibronectin、RHAMM和Laminin基因的表达,但当bIFN-τ和孕酮联合作用时,CD44、THBS基因的转录水平受到抑制,Tenascin、Fibonectin、Colla-gen基因的转录水平恢复到对照组转录水平。研究结果显示,在胚胎植入过程中,IFN-τ和孕酮可能对牛子宫内膜上ECM及其配基基因表达具有调控作用。

To reveal the effects of interferon τ（IFN-τ） and progesterone on the expression level of extracellular matrix（ECM） and their ligands genes in bovine endometrial cells（bECs）.In the study,bECs were cultured in medium containing bovine IFN-τ（bIFN-τ）,progesterone and combination of bIFN-τ and progesterone,respectively,and then expression level of ECM related genes（Collagen,laminin,Fibronectin,THBS and Tenascin） and their ligand genes（Syndecan,CD44,CD47 and RHAMM） in bECs were detected by quantitative PCR after in vitro cultured bECs were treated for 24 h with bIFN-τ,progesterone,combination of bIFN-τ and progesterone,respectively.The results showed that,bIFN-τ significantly induce the expression of Tenascin,Collagen,RHAMM and Laminin,and progesterone significantly induced the expression of CD44,Fibronectin,RHAMM,and Laminin,but under co-action of bIFN-τ and progesterone,the expression level of CD44 and THBS was inhibited,and the expression of Tenascin,Fibonectin and Collagen returned to the expression level in the control group.The results suggest that bIFN-τ and progesterone might regulate the expression of ECM and their ligands in the process of embryo implantation.