枸杞多糖对雏鸡淋巴细胞体外增殖及分泌IL-2的影响

Effects of Lycium barbarum Polysaccharide on Cell Proliferation of Chicken Lymphocytes and IL-2 Excretion in vitro

为探讨枸杞多糖(LBP)对雏鸡T、B淋巴细胞体外增殖的影响,将不同浓度的LBP分别加入到用植物血凝素(PHA)刺激的体外培养的雏鸡胸腺、外周血、脾脏淋巴细胞中,以及用细菌脂多糖(LPS)刺激的体外培养的雏鸡法氏囊、外周血、脾脏淋巴细胞中,分别用MTT比色法和ELISA法测定淋巴细胞增殖的变化以及IL-2的分泌量。结果显示:(1)LBP在一定浓度范围内时,均能刺激T、B淋巴细胞的增殖和促进IL-2的分泌,且随着多糖浓度的增加而增加;(2)LBP对同一器官来源的T、B淋巴细胞的OD值随着雏鸡日龄的增长均呈现增长趋势;(3)同浓度LBP对相同日龄雏鸡胸腺T淋巴细胞和法氏囊B淋巴细胞的OD值均高于外周血和脾脏的T、B淋巴细胞OD值。结果表明LBP能够促进雏鸡淋巴细胞的增殖和IL-2的分泌,并且对淋巴细胞增殖的促进作用有一定的量效、时效关系和组织相关性。

This experiment was conducted to investigate the effects of Lycium barbarum polysaccharide（LBP） on cell proliferation of chicken Lymphocytes in vitro.Different concentrations of LBP were added into cultured with plants hemagg-lutinin（PHA） stimulated chicken thymus,spleen and peripheral blood lymphocyte,and cultured with bacteria lipopolysaccharide（LPS） stimulated chicken bursa of Fabricius,peripheral blood lymphocyte,spleen,MTT method and ELISA method were used to determine the lymphocyte proliferation and IL-2 excretion respectively.The results showed that：（1） LBP could stimulate T,B lymphocyte proliferation and IL-2 excretion in certain concentration range and with the increase of the concentration of polysaccharide increase;（2） The OD value of T,B lymphocyte with the source of the same organ were significantly growth trend with chickens dayage growth in same concentration of LBP.（3） The OD value of thymus T lymphocyte and bursa of Fabricius B lymphocyte were higher than in peripheral blood and the spleen T,B lymphocytes with the same concentration LBP and the same day age of chicken.These results indicated that LBP could stimulate T,B lymphocyte proliferation and IL-2 excretion,and the promoting function of lymphocyte proliferation have certain concentration-response,time relationship and organizational correlation.