与人遗传病相关的DNA重复序列的体外核小体定位特性

In vitro nucleosome positioning features of DNA repeats sequence associated with human genetic disease

目的研究与人遗传病相关的DNA重复序列的体外核小体定位。方法构建含有(GAA)42、(ATTCT)43、(GCCT)18和601序列的重组质粒,体外利用盐透析将质粒与组蛋白八聚体组装形成染色质结构,微球菌核酸酶消化后,用琼脂糖凝胶电泳分析染色质的结构。结果含有ATTCT重复序列的质粒较含GAA重复序列质粒在体外易于形成核小体。结论在重组质粒中,由于引入的重复序列片段形成核小体能力的不同会影响其局部染色质结构。

Objective To investigate the nucleosome positioning of DNA repeats sequence in vitro which can cause human genetic disease. Methods The recombinant plasmids containing （GAA） 42, （ATTCT）43, （GCCT）18 and 601 sequence were cloned.The histone and plasmids were used to assemble chromatin structure in vitro,and then analyzed by agarose gel electrophoresis after micrococcal nuclease digestion. Results The plasmid containing ATTCT repeats sequence was more easy to form nucleosome than containing GAA repeats sequence in vitro. Conclusion The recombinant plasmids’ ability to form chromatin structure were changed because of the insert of the different repeats sequence fragment.