盘基网柄菌细胞黏附分子cadA/DdCAD-1在细胞分化及细胞命运决定中的作用

Dictyostelium discoideum Cell Adhesion Molecule cadA/DdCAD-1 Plays An Important Role in Cell Differentiation and Cell Fate Determination

盘基网柄菌细胞黏附分子DdCAD-1是在细胞发育过程中最先表达的黏附分子,为了研究DdCAD-1在盘基网柄菌细胞发育中的作用,将cadA基因的突变株cadA-细胞用中性红染料染色,发育成的蛞蝓体显示cadA-细胞的前柄细胞/前孢子细胞的分化出现明显的障碍,外源表达的重组蛋白His6-DdCAD-1与cadA-细胞作用一段时间后,这种现象得到了改善。另外,cadA-细胞的孢子产率也有所降低,外源重组蛋白也可以拯救该表现型。表达DdCAD-1的细胞与cadA-细胞共同发育所形成的嵌合体显示表达DdCAD-1的细胞占据在拔顶期结构的顶端及尾部,而这些结构都在非孢子区,最终会死亡。提示DdCAD-1对于细胞分化及细胞命运决定有重要意义。

Cell adhesion molecule cadA/DdCAD-1 is the first expressed adhesion molecule during the development of Dictyostelium discoideum, to investigate the role of DdCAD-1 in D. discoideum development, cadA mutant strain cadAcells were colored by Natural Red, slug structure show obviously aberrant cell differentiation. Exogenous recombinant protein His6-DdCAD-1 rescued this phenomenon. Additionally, recombinant protein can also rescue the decreasing spore yield of cadA- cells. When DdCAD-1 expressed cells were mixed with cadA-cells, DdCAD-1 expressed cells occupied the tips and posterior region of the chimeric culmination structures, which do not belong to spore area and would die eventually. All these results suggest that DdCAD-1 plays a crucial role in cell differentiation and cell fate determination.