肿瘤微环境对骨髓间充质干细胞形态、生长及增殖的影响

The Effect of Tumor Microenvironment to Morphology,Growth and Proliferation of Human Mesenchymal Stem Cells-bone Marrow

因骨髓间充质干细胞(human mesenchymal stem cells-bone marrow,HMSC-bm)易于体外分离、培养、扩增及外源基因的导入,肿瘤趋向性和低免疫源性等特点,近年来已成为肿瘤生物治疗理想的载体。该实验采用Transwell小室建立HMSC-bm与肺腺癌细胞A549非接触共培养体系,研究肿瘤微环境诱导3 d、7 d后分别传至第3代、第5代HMSC-bm相关生物学特性的改变。结果显示,随着共培养时间的延长及传代代次的增加,实验组HMSC-bm细胞形态逐渐发生显著变化;细胞生长曲线与周期检测结果发现细胞增殖速度逐渐增快,提示肺腺癌微环境诱导HMSC-bm分化,导致其形态、生长及增殖等生物学特性改变,具有向肿瘤转化的可能,但尚需进一步研究,为HMSC-bm的临床广泛应用提供科学依据。

Because of the advantages of human mesenchymal stem cells-bone marrow （HMSC-bm） such as easy to be isolated, cultured, expanded in vitro and imported exogenous gene, the tropism to tumor, the lowdown immunogenicity etc., it becomes an ideal target therapeutic vector for tumor＇s biological treatment in recent years. In this study, a co-culture system of HMSC-bm and lung adenocarcinoma cell line A549 was established by using Transwell chamber. To study the related biological characteristics＇ changes of HMSC-bm, induced for 3 and 7 days, and then, passaged to the third generation and the fifth generation respectively in tumor microenvironment. The results showed that along with the extension of co-culture time and the increase of the passage, morphology of the experimental groups cells changed gradually. MTT assay and Cell cycle analysis indicated that the experimentalgroups cells growth vigor was reinforced gradually. The results suggested HMSC-bm was induced to differentiate in lung adenocarcinoma microenvironment, its biological characteristics such as morphology, growth and proliferation were changed. The HMSC-bm may be has tumorigenicity. But the mechanism of differentiation should be studied deeply to provide the scientific evidence for widely clinical application of HMSC-bm.