降血尿酸乳酸菌筛选及其对高尿酸血症模型大鼠作用研究

Screening of serum uric acid-lowering Lactic acid bacteria and its effect on hyperuricemia in rat models

目的提出利用具特殊活性乳酸菌来预防、治疗高尿酸血症方法,筛选具有较高降血尿酸活性的乳酸菌。方法基于RP-HPLC方法,通过检测培养液培养前后肌苷、鸟苷含量变化,筛选出具有最快核苷分解速率的乳酸菌株作为候选菌株。并将其施用于高尿酸血症模型大鼠,观察对模型大鼠血尿酸含量影响。结果获得具有最佳核苷分解速率的菌株DM9218。动物实验结果表明DM9218干预组的血尿酸浓度(219.25±21.98)μmol/L明显低于模型组(311.75±27.07)μmol/L(P<0.05)。结论菌株DM9218具有最快的核苷分解速率,对高尿酸血症大鼠具有明显的降血尿酸作用。菌株DM9218为植物乳杆菌(Lactobacillus plantarum)。

Objective To propose a method for the prevention and treatment of hyperuricemia by Lactic acid bacteria（LAB） with specific activity,and screen LAB strains with high serum uric acid-lowering activity.Methods Based on RP-HPLC method,the changes of inosine and guanosine contents in the culture medium before and after cultivation were determined;The LAB strains which presented the fastest rates of decomposition of nucleosides were selected as the candidate strains,and administered to the model rats.The effect of the strains on the serum uric acid level of the rats were observed.Results The strain DM9218 with an optimal decomposition rate of nucleoside was obtained.Animal experimental results showed that the serum uric acid level of DM9218 intervention group [（219.25 ± 21.98） μmol/L] was obviously lower than that of the model group [（311.75 ± 27.07） μmol/L]（P〈0.05）.Conclusion DM9218 has the fastest decomposition rate of nucleosides and obvious effect of lowering serum uric acid in hyperuricemia rats.DM9218 is identified as Lactobacillus plantarum.