SLE患者外周血CD3^+ CD16^+ CD56^+ NK T细胞和CD3^+ CD4^- CD8^- DN T细胞的流式细胞分析及意义

Analysis and significance of CD3^+ CD16^+ CD56^+ natural killer T cells and CD3^+ CD4^- CD8^- double negative T cells from peripheral blood using flow cytometry in patients with systemic lupus erythematosus

目的探讨CD3+CD16+CD56+天然杀伤T(NK T)细胞和CD3+CD4-CD8-双阴性T(DN T)细胞在SLE患者外周血中的变化及意义。方法选取32例SLE活动期患者作为SLE活动组、30例SLE稳定期患者作为SLE稳定组、25例健康人作为健康人对照组,用流式细胞术检测各组对象外周血中NK T细胞和DN T细胞的比率,并比较其与SLE疾病活动性指数(SLEDAI)之间的相关性。结果 SLE活动组外周血NK T细胞比率(3.47±1.49)%显著低于SLE稳定组(5.30±1.83)%及健康人对照组(6.24±2.11)%,P均<0.01,但SLE稳定组与健康人对照组差异无统计学意义(P>0.05);SLE活动组外周血DN T细胞比率(5.16±1.97)%显著高于SLE稳定组(4.00±1.37)%及健康人对照组(3.15±1.06)%,P均<0.01,SLE稳定组亦高于健康人对照组(P<0.05)。SLE患者外周血NK T细胞比率与SLE DAI呈显著负相关(r=-0.439,P<0.01);SLE患者外周血DN T细胞比率与SLE DAI呈显著正相关(r=0.446,P<0.01);SLE患者外周血NK T细胞比率与DN T细胞比率之间无明显相关性(r=-0.215,P>0.05)。结论外周血中NK T细胞及DN T细胞比率改变可能参与了SLE的发病过程,且可能与SLE的病情活动性相关。

Objective To investigate the changes and significance of CD3＋CD16＋CD56＋ natural killer T cells（NK T） and CD3＋CD4-CD8-double negative T cells（DN T） in the peripheral blood of patients with systemic lupus erythematosus（SLE）.Methods The percentages of NK T and DN T cells in the peripheral blood of 32 patients in active stage of SLE,30 patients in stable stage of SLE and 25 healthy controls were detected by flow cytometry.The correlations of NK T cells and DN T cells with SLE DAI were analyzed.Results The percentage of NK T cells in the peripheral blood of the patients in active stage was lower than that of stable stage patients and healthy controls（P0.01）,and there was no difference with statistical significance between stable stage and healthy controls（P0.05）.The percentage of DN T cells in the peripheral blood of active stage patients was higher than that of stable stage patients and healthy controls（P0.01）,but there was a difference with statistical significance between stable stage patients and healthy controls（P0.05）.The percentage of NK T cells was negatively correlated with SLE DAI（r=-0.439,P0.01）,while the percentage of DN T cells was positively correlated with SLE DAI（r=0.446,P0.01）.There was no correlation of percentage between NK T and DN T cells（r=-0.215,P0.05）.Conclusion The changes of NK T cells and DN T cells in the peripheral blood might contribute to the pathogenesis and correlate with the disease activity of SLE.