摘要
目的探讨耐药克雷伯属菌获得性耐药相关基因和可移动遗传元件的关系以及与菌株间的亲缘关系。方法收集20株从患者标本中分离的耐药克雷伯属菌,用PCR法分析67种水平转移获得的β-内酰胺类、氨基糖苷类、喹诺酮类获得性耐药基因和12种接合性质粒、转座子、插入序列、整合子等可移动元件标志基因,再对检测结果作指标聚类分析(UPGMA法)和样本聚类分析(neighbour-joining法)。结果 20株耐药克雷伯属菌共检出6种β-内酰胺类、5种氨基糖苷类、4种喹诺酮类耐药基因和8种可移动遗传元件的遗传标志;指标聚类分析提示,KPC与ISKpn6高度相关联,SHV与IS26高度相关联;样本聚类分析显示,4号与18号株、15号与20号株分别为克隆传播。结论克雷伯属菌耐药元件检测可揭示菌株耐药的遗传学背景。
Objective To investigate the correlation between acquired drug resistance genes and mobile genetic elements in drug-resistant Klebsiella and their phylogenetic relationship.Methods Twenty strains of drug-resistant Klebsiella were collected from patients′ samples.Then,67 kinds of acquired drug resistance genes,including beta-lactams,aminoglycosides and quinolones,and 12 kinds of mobile genetic elements,such as conjugal plasmids,transposons,insertion sequences and integrons,were detected by PCR.Finally,the determination results were performed index cluster analysis(by UPGMA method) and sample cluster analysis(by neighbour-joining method).Results Six kinds of beta-lactam-resistance genes,5 aminoglycoside-resistance genes,4 quinolone-resistance genes and 8 kinds of mobile genetic elements were detected from 20 strains of drug-resistant Klebsiella.Index cluster analysis showed that there were high correlations between KPC and ISKpn6,and between SHV and IS26.ASample cluster analysis showed that strains No.4 and No.18,and strains No.15 and No.20 belonged to the same clone,respectively.Conclusion The detection of drug resistance genes and mobile genetic elements in drug-resistant Klebsiella may reveal the genetic background of these strains to resist drugs.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2013年第1期32-35,共4页
Chinese Journal of Clinical Laboratory Science
基金
淮安市科技基金项目(HAS2010022)