MGF-Ct24E改性聚乳酸的合成、表征及其对成骨细胞增殖的评价

Synthesis,characterization and osteoblasts proliferation of a novel biomimetic material based on MGF-Ct24E modified poly(D,L-lactic acid)

力生长因子(MGF)是骨修复重建的一种重要生长因子。以二环己基碳二亚胺为缩合剂,将MGF羧基端E结构域24肽(MGF-Ct24E)共价接枝到丁二胺改性的聚乳酸上(DPLA),制得了新型MGF-Ct24E改性聚乳酸仿生材料(MGF-Ct24E-DPLA)。采用氨基酸分析和高效液相色谱对MGF-Ct24E含量进行了定性定量表征,静态水接触角和吸水率测定了MGF-Ct24E-DPLA材料的亲水性,MTT法评价了其对成骨细胞的增殖作用。结果表明,MGF-Ct24E成功引入到DPLA中,接枝效率为24.7%,并且和DPLA相比,MGF-Ct24E-DPLA材料具有更好的亲水性和促进成骨细胞增殖的能力。因此这种新型MGF-Ct24E改性聚乳酸仿生材料有望成为骨组织工程领域中一种卓越的生物材料。

Mechano-growth factor（MGF） is one of the most important growth factors of bone regeneration.A novel biomimetic poly（D,L-lactic acid）（PDLLA） modification was designed and synthesized based on MGF-Ct24E grafted butanediamine modified PDLLA（DPLA）.MGF-Ct24Es were grafted into the side chain of DPLA via a stable covalent amide bond by using dicyclohexylcarbodiimide（DCC） as the condensing agent to produce biomimetic DPLA materials（MGF-Ct24E-DPLA）.Amino acid analyzer（AAA） and high performance liquid chromatography（HPLC） were used to characterize the MGF-Ct24E-DPLA.The hydrophilicity of MGF-Ct24E-DPLA was evaluated by means of the water-uptake ratios and static water contact angle.Data revealed that the grafting efficiency of MGF-Ct24E was about 24.7%.MGF-Ct24E-MPLA had better hydrophilicity than DPLA.The osteoblasts behavior of proliferation,on glass,DPLA and MGF-Ct24E-DPLA films was investigated and the results indicated that the introduction of MGF-Ct24E could improve osteoblasts proliferation.The MGF-Ct24E-MPLA with higher bioactivity may have potential application for bone tissue engineering.