聚氨酯/纳米羟基磷灰石/聚酰胺66股骨髁修复犬骨软骨缺损的实验研究

In vivo study on polyurethane/nano-hydroxyapatitie/polyamid66 femoral condyle for reparing articular osteochondral defects for femur in dog

制备了PU/n-HA/PA66股骨髁,用扫描电镜观察材料表面情况并测定其孔隙率;将PU/n-HA/PA66股骨髁与自体髁植入犬股骨远端,以替代、修复骨软骨缺损,进行大体观察、组织学、免疫组化、CT、血常规和生化检测以及肝、肾、脾组织学检测。结果表明材料孔隙率为80.89%±5.01%,孔径主要分布在300～800μm之间。术后实验动物活动正常,切口愈合良好,两组髁假体均与自体骨结合紧密,PU/n-HA/PA66股骨髁网孔中的骨小梁逐渐增多成熟,材料孔穴中的新生骨Ⅰ型胶原阳性表达。术后动物碱性磷酸酶水平升高[(62.67±24.04)U/L],肝、脾、肾HE染色未见异常。PU/n-HA/PA66股骨髁具有良好的骨修复、软骨替代能力和生物相容性,具有应用前景。

The PU/n-HA/PA66 femoral condyle was prepared.Material surface was observed with scanning electron microscope and the porosity was measured.The PU/n-HA/PA66 femoral condyle and the autologous femoral condyle were implanted into the distal femur and the articular cartilage and subchondral bone defects were repaired with PU/n-HA/PA66 femoral condyle and autologous femoral condyle separately.Gross examination,histological staining,collagen Ⅰ immunohistochemical staining,CT examination,blood routine and serum biochemical index and histological staining of the liver,kidney and spleen were conducted 24 weeks after operation.The porosity of the composite was 80.89%±5.01% and main pore size reached from 300μm to 800μm.The activity of dogs was normal and the cut healed well in both groups after operation.CT examination,histological investigation and immunohistochemical staining showed the two kinds of condylar prosthesises were closely combined with autologous bone in all the experiment animals.Trabecular bone in the pores of the biological femoral condyle became more and more and calcified gradually into mature bony tissue.The expression of collagen Ⅰ of newly formed bone in the pores of materials was positive.The blood routine and serum biochemical index were both normal except the level of alkaline phosphatase increased[（62.67±24.04）U/L] after operation.Histological staining of the liver,kidney and spleen was normal 24 weeks after operation.PU/n-HA/PA66 femoral condyle has good internal bone defects repairing ability,cartilage substitution ability and biocompatibility,which is suitable for orthopedic biomaterials.