温敏型P(NIPA-co-EA)线性共聚物的合成及性质

Synthesis and properties of thermosensitive linear copolymer of P(NIPA-co-EA)

利用N-异丙基丙烯酰胺(NIPA)以及N-乙基丙烯酰胺(EA)两种单体合成了温敏型线性共聚物P(NIPA-co-EA),并用核磁共振氢谱、红外光谱以及凝胶渗透色谱(GPC)对该聚合物的结构进行了表征。采用浊度观察法和分光光度法两种方法分别对聚合物的LCST值进行了测试研究,探讨了通过调节两种单体的组分比例来调控体系的LCST值,并讨论了外添加剂NaCl以及BSA对线性聚合物P(NIPA-co-EA)LCST的影响。结果表明,随着EA单体摩尔配比的增大,对应共聚物的LCST值也相应增大,且在32～41.6℃之间可调。向P(NIPA-co-EA)水溶液中加入NaCl,当NaCl的量由0.9%(质量分数)增加到2%(质量分数)时,共聚物的LCST值从34.3℃降为31.6℃;加入BSA,线性共聚物的LCST随BSA浓度的增加而增加,且当EA10水溶液中加入20mg/mLBSA时,线性共聚物P(NIPA-co-EA)的LCST可高达38.9℃。

A novel therosensitive copolymer P（NIPA-co-EA） was synthesized based on the monomer of N-isopropylacrylamide（NIPA） and N-ethylacrylamide（EA）.1H-NMR spectra,IR and GPC were employed to characterize the structure of the polymer.The LCST value of P（NIPA-co-EA） was adjusted by the component ratio of the two monomers and measured by turbidity and spectrophotometer methods.Moreover,the influence of additives to the LCST value of linear copolymer P（NIPA-co-EA）,NaCl and BSA were studied.The results indicated that the LSCT value of copolymer P（NIPA-co-EA） was increased along with the increasing of molar ratio of EA monomer and it could be adjusted between 32.0-41.6℃.When the amount of NaCl was changed from 0.9wt% to 2wt% and added into P（NIPA-co-EA） solution,the LCST value of copolymer P（NIPA-co-EA） was decreased from 34.3℃ to 31.6℃.However,the increased concentration of BSA adding into P（NIPA-co-EA） solution made the LCST valued rised.What＇s more,when 20 mg/mL BSA was added into EA10 solution,the LCST value of copolymer P（NIPA-co-EA） could up to 38.9℃.