同步辐射显微红外光谱研究6-OHDA诱导帕金森病细胞模型

Synchrotron Radiation-Based FTIR Microspectroscopy Study of 6-Hydroxydopamine Induced Parkinson's Disease Cell Model

神经毒素6-hydroxydopamine(6-OHDA)处理的人神经瘤母细胞系SH-SY5Y是一种经典的帕金森病细胞模型。利用同步辐射红外显微光谱研究6-OHDA处理的SH-SY5Y细胞系的生物化学成分。与正常细胞相比较,所含磷脂的平均饱和水平显著提高,蛋白质的二级结构发生显著变化,其中的β-sheet的比例明显增高,核酸的含量明显下降,提示神经毒素6-OHDA对细胞造成了严重的氧化损伤。同步辐射红外显微光谱能够精确有效的检测到细胞生化成分的改变,从而对细胞的病理损伤进行评估。

SH-SY5Y cell line treated with 6-hydroxydopamine（6-OHDA） is a classical Parkinson＇s disease（PD）.In the present study,synchrotron-based Fourier transform infrared（FTIR） microspectroscopy was used to analyze the biochemical composition of SH-SY5Y cell line treated with 6-OHDA.The detailed spectral analyses show the significant changes in cellular compositions such as lipids,proteins and nucleic acids in SH-SY5Y cells treated with 6-OHDA compared to control SH-SY5Y cells.As a result,the unsaturation levels of phospholipids decrease in SH-SY5Y cells treated with 6-OHDA compared to control cells,the analysis of protein secondary structure shows the significantly higher ratio of β-sheet in PD cells compared to that of control cells,and the content of nuclear acid is highly decreased compared to that of control cells,suggesting that 6-OHDA induces the serious oxidative damage in SH-SY5Y cells.These findings suggest that SR-FTIR is an effective and precise technical tool to probe the biochemical changes of cells and then evaluate the pathological damage in cells.