绿豆多糖制备及抗氧化特性研究

Study on the Preparation of Polysaccharides from Mung Bean Seed and Anti-Oxidant Activity

采用响应面法对绿豆仁中多糖碱液提取工艺进行优化,在此基础上进一步开展分离纯化及抗氧化特性研究。结果表明,碱法提取绿豆多糖(Alkali extraction mung bean polysaccharides,AEMP)最佳工艺参数为碱液浓度0.02 mol/L,液料比20∶1 mL/g,提取温度50℃,提取时间3 h,最终AEMP得率9.70 mg GE/g DW。进一步采用DEAE-2纤维柱和SephadexG-100凝胶柱分离纯化绿豆多糖,得到一个中性组分AEMP-1和一个酸性组分AEMP-2。抗氧化特性结果表明AEMP及其纯化组分对羟自由基和DPPH自由基均有良好清除效果,AEMP-2抗氧化活性最强,对羟自由基和DPPH自由基半数抑制浓度IC50值分别为4.71 mg/mL和1.03×10-1mg/mL。表明绿豆多糖具有良好的抗氧化特性。

Response surface methodology （RSM） alkali extraction on the yield of polysaccharides, and on was used to optimize the effects of processing parameters of the basis of which, anti - oxidative activity was investigated The optimal extraction condition of polysaccharides was determined as follows with Alkali extraction mung bean poly-saccharides （AEMP） ：concentration of alkali solution was 0.02 mol/L, ratio of water to raw material was 20：1 mL/g, extraction temperature was 50℃ and treatment time was 3.0 h. Under this condition, the experimental recovery of crude alkali extraction mung bean polysaccharides （AEMP） was 9.70 mg GE/g DW. AEMP was purified by sepha- dex G - 100 gel chromatogarph after anion - exchange DEAE - 52 cellulose chromatograph. A neutral fraction （ AEMP - 1 ） and one acid fraction （ AEMP -2） were gained. The result of anti - oxidative activity showed that AEMP and its purified components showed excellent scavenging activity on the hydroxyl radicals and a, a - diphenyl - 1 - picryl- hydrazyl （DPPH） radicals. AEMP - 2 had best radicals scavenging activity, and ICso values was 4.71 mg/mL （ hy- droxyl radicals） and 1.03×10^-1 mg/mL （DPPH）, respectively. Therefore, mung bean polysaccharides have a great anti - Oxidant activity.