慢性乙型肝炎病人NK细胞细胞毒活性的研究

AN INVESTIGATION OF NATURAL KILLER CELL MEDIATED CYTOTOXICITY IN PATIENTS WITH CHRONIC HEPATITIS B

本文应用3H-TdR释放法检测22例慢性乙型肝炎病人外周血NK细胞细胞毒活性。并以24名正常人作对照。结果:慢活肝12例及慢迁肝10例的NK细胞毒活性(x±s),分别为48.1±20.9％及32.6±9.8％,和对照组23.1±19.5％比较,P值分别<0.002和<0.05。而慢活肝亦较慢迁肝组增高(P值<0.05)。上述结果提示,慢活肝和慢迁肝病人NK细胞活性均较正常人增高。而肝脏病变较重的慢活肝较病变较轻的慢迁肝增高。NK细胞活性和肝细胞损伤及损伤程度一致。NK细胞可能参与慢乙肝肝细胞的损伤机制。

We used ~3H-TdR released method to detect the activity of Natural killer(NK) cell mediatedcytotoxicity in 22 patients with chronic hepatitis B, included chronic active hepatitis (CAH) 12 casesand chronic persistent hepatitis (CPH) 10 cases. All cases were proved by liver biopsy. We alsoinvestigated 24 normal healthy persons as control. The results showed that the activity of NK cellmediated cytotoxicity in patients with CAH and CPH were 48.1±20.9% and 32.6±9.8% respectivelyNK cell activity in normal persons was 23.1±19.5%, P<0.002 and<0.05 respectively, the activityof NK cell in patients with CAH was higher than that of patients with CPH, P<0.05. It suggestedthat NK cell activity in chronic hepatitis B included CAH and CPH were higher than that of normalpersons, especially in CAH. It also suggested that the activity of NK cell mediated cytotoxicity wascompatible with liver cell injury, it was probably involved in the mechanism of liver cell injury inviral hepatitis B.