P188在电损伤动物实验中疗效观察的研究

The effect of P188(poloxamer) in animal experiment after electrical trauma

目的通过动物实验了解P188在电损伤后对全身及局部组织的影响。方法将30只新西兰大白兔随机分为3组。即P188组、P188+Vc组和盐水组。建立高压电的发电装置。电击后在不同的时间段内静脉输入不同的液体。最后静脉采血获取各项生化指标。对各项实验数据进行统计分析,并取不同的组织标本进行病理检测。结果家兔在电击后血浆中SGPT(85±17)U/L明显高于电击前的正常对照值(52±15)U/L,与P188+Vc组比较P值均<0.01。乳酸脱氢酶(850±42)U/L也明显高于正常对照值(340±45)U/L,并与P188组、P188+Vc组比较P值均<0.01。血清肌酸激酶含量电击后为(316±20)U/L,明显高于正常值(243±48)U/L,并与P188组及P188+Vc组比较P值均<0.01。血浆中纤维蛋白原的含量电击后为(5.41±0.7)μg/L,高于正常值(3.76±0.8)g/L,与P188组及P188+Vc组比较均有极显著性差异。血浆中的D-二聚体的含量电击后为(198±18)μg/L,明显高于正常值(144±32)μg/L,与P188组及P188+Vc组比较P值均<0.01。结果显示在使用P188后血浆生化指标均有不用程度的改善。在电击后肌肉出现间质水肿、部分肌纤维肿胀出现变性坏死、神经部分细胞也出现变性。在使用P188后均未出现明显异常。结论 P188在实验兔电损伤后对全身及局部损伤组织具有积极的修复作用。

Objective To study the effect of P188 （poloxamer） in animals after electrical trauma. Methods Electrical trauma model was reproduced to 30 New Zealand rabbits. They were randomly divided into three groups ：P188 group,P188＋Vc group, and saline group （as control group）. Various liquids was given at different times after electrical trauma.Vein blood taken from the rabbits both before and after electrical trauma was given for the correlative biochemistry index. Parts of the tissue sam- pies were examined pathologically. Data were processed with analysis of variance. Results In all three groups the contents of SGPT, fibrinogen, activities of LDH , and CPK in plasma after electrical trauma were obviously higher than those before the truama, P〈0.01. The contents of D-dimer in P188 group and P188 ＋Vc group were also obviously lower than control group （144±32） μg/L. The results above showed a very important significance. Muscle interstitial edema, swelling of muscle,degenera- tion of nerve cell occured after electrical trauma. Conclusion There is advantaged effect of P188 on rabbits after electrical trauma.