HPLC-ELSD测定丹参提取物中的单糖和二糖

Determination of Monosaccharide and Disaccharide in Tanshinon Extract by HPLC-ELSD

目的建立一种以高效液相色谱-蒸发光散射（HPLC-ELSD）对丹参提取物中单糖和二糖进行测定的方法。方法使用PrevailTM Carbohydrate ES色谱柱,以乙腈和水为流动相,梯度洗脱,柱温为35℃,流速为0.8 mL.min 1;ELSD漂移管温度为60℃,载气为空气,气压为25 psi。结果丹参提取物中单糖和二糖组分能够得到较好的分离,葡萄糖、果糖和蔗糖的线性范围分别为0.246-1.230,0.236-1.182,0.246-1.230 mg;平均加样回收率（n=6）分别为101.7%（RSD=1.6%）、102.0%（RSD=1.0%）和100.8%（RSD=0.9%）。结论该方法快速简捷、结果准确稳定,适合于丹参提取物中单糖、双糖的含量测定。

OBJECTIVE An HPLC-ELSD method was established for the simultaneous quantitative analysis of monosaccharide, disaccharide from Tanshinon extract. METHODS The chromatographic separation was achieved on PrevailTM Carbohydrate ES column using a mobile phase composed of a mixture of acetonitrile and water by gradient elution at a flow rate of 0.8 mL.min-1. The ELSD drift tube temperature was set at 60 ℃ and the carrying gas was air. RESULTS Monosaccharides and disaccharides from Tanshinon extract could be well separated, and the linear ranges of fructose, glucose and sucrose were 0.246-1.230 mg, 0.236-1.182 mg and 0.246-1.230 rag. The average spiked recoveries（n=6） of fructose, glucose and sucrose were 101.7%（RSD=1.6%）, 102.0%（RSD=1.0%） and 100.8%（RSD=0.9%）. CONCLUSION This method is accurate, rapid, simple and reproducible, and thus it is suitable for the determination of monosaccharides, disaccharides from Tanshinon extract.