超高效液相色谱-串联质谱测定保健食品中马兜铃酸A

Determination of Aristolochic Acid A in Health Food by UHPLC-MS/MS

目的建立测定保健食品中马兜铃酸A的超高效液相色谱-串联质谱法。方法采用Zorbax Eclipse Plus C18（2.1 mm×100 mm,1.8μm）;流动相为乙腈-0.1%甲酸（含10 mmol.L 1甲酸铵）（60∶40）;流速：0.2 mL.min 1;质谱条件为电喷雾电离源（ESI＋）,以多重反应监测（MRM）方式进行检测,用于定量分析的反应离子为m/z 359→324。结果马兜铃酸A在2~200 ng.mL 1内线性关系良好,方法平均回收率为105.3%,RSD为0.8%,最低检测限为0.2μg.kg 1。结论本法专属性强,灵敏度和准确度高,可用于保健食品中马兜铃酸A的测定。

OBJECTIVE To establish a UHPLC-MS/MS method for the determination of aristolochic acid A in health food. METHODS The separation and analysis was performed on a Zorbax Eclipse Plus C18 column（2.1 mm×100 mm, 1.8 μm）. The mobile phase consisted of acetonitrile-0.1% fomic acid containing 10 mmol.L-1 ammonium formate（60 ： 40）. The flow rate was 0.2 mL.min-1. Electrospray ionization（ESI） source was applied and operated in the positive mode. Multiple reaction monitoring （MRM） mode with the transitions of m/z 359±324 was used to quantify aristolochic acid A. RESULTS The assay linearity of aristolochic acid A was confirmed over the range 2-200 ng.mL-1. The average recovery of aristolochic acid A was 105.3% and RSD was 0.8%. The detection limit was 0.2 μg-kg-1. CONCLUSION The method is proved to be specific, sensitive and accurate. Therefore. it can be used to detect aristolochic acid A in health food.