摘要
目的建立HPLC-ELSD测定益母草颗粒中盐酸水苏碱的含量。方法色谱柱为Venusil HILIC丙基酰胺键合硅胶柱(250 mm×4.6 mm,5μm);柱温为25℃;流动相为乙腈-0.2%冰醋酸溶液(80∶20);流速为0.8 mL.min 1;进样量为10μL;漂移管温度为80℃,氮气流速为1 L.min-1。结果盐酸水苏碱在0.646 5-12.93μg内呈良好的线性关系,r=0.999 4(n=5);平均加样回收率为98.48%,RSD=1.15%(n=9)。结论该方法简便、快速、准确、重复性和稳定性好,可作为益母草颗粒的盐酸水苏碱含量测定方法。
OBJECTIVE To establish an HPLC-ELSD method for the determination of stachydrine hydrochloride content in Herba leonuri granule. METHODS The Venusil HILIC column(250 mmx4.6 mm, 5 μm) was adopted. The column temperature was 25℃. The mobile phase was acetonitrile-0.2% glacial acetic acid(80 : 20). The flow rate was 0.8 mL.min-1. The injection volume was 10 gL. The drift tube temperature was 80 ℃, and the nebulizer gas flow rate was 1 L.min-1. RESULTS The linear range of staehydrine hydrochloride was 0.646 5-12.93 μg, r=0.999 4(n=5). The average recovery was 98.48%, RSD=1.15%(n=9). CONCLUSION The HPLC-ELSD method is convenient, rapid and accurate with good reproducibility and stability which is suitable for assay of stachydrine hydrochloride content in leonuri granule.
出处
《中国现代应用药学》
CAS
CSCD
2013年第2期189-191,共3页
Chinese Journal of Modern Applied Pharmacy