HPLC-ELSD测定益母草颗粒中盐酸水苏碱的含量

Determination of Stachydrine Hydrochloride in Leonuri Granule by HPLC-ELSD

目的建立HPLC-ELSD测定益母草颗粒中盐酸水苏碱的含量。方法色谱柱为Venusil HILIC丙基酰胺键合硅胶柱（250 mm×4.6 mm,5μm）;柱温为25℃;流动相为乙腈-0.2%冰醋酸溶液（80∶20）;流速为0.8 mL.min 1;进样量为10μL;漂移管温度为80℃,氮气流速为1 L.min-1。结果盐酸水苏碱在0.646 5-12.93μg内呈良好的线性关系,r=0.999 4（n=5）;平均加样回收率为98.48%,RSD=1.15%（n=9）。结论该方法简便、快速、准确、重复性和稳定性好,可作为益母草颗粒的盐酸水苏碱含量测定方法。

OBJECTIVE To establish an HPLC-ELSD method for the determination of stachydrine hydrochloride content in Herba leonuri granule. METHODS The Venusil HILIC column（250 mmx4.6 mm, 5 μm） was adopted. The column temperature was 25℃. The mobile phase was acetonitrile-0.2% glacial acetic acid（80 ： 20）. The flow rate was 0.8 mL.min-1. The injection volume was 10 gL. The drift tube temperature was 80 ℃, and the nebulizer gas flow rate was 1 L.min-1. RESULTS The linear range of staehydrine hydrochloride was 0.646 5-12.93 μg, r=0.999 4（n=5）. The average recovery was 98.48%, RSD=1.15%（n=9）. CONCLUSION The HPLC-ELSD method is convenient, rapid and accurate with good reproducibility and stability which is suitable for assay of stachydrine hydrochloride content in leonuri granule.