牛病毒性腹泻病毒山东株的分离与鉴定

Isolation and Identification of Bovine Viral Diarrhea Virus Shandong Strain

牛病毒性腹泻病毒是牛病毒性腹泻-黏膜病的重要病原体,该病毒病是极为复杂、呈多临床类型表现的传染病,给世界养牛业造成了巨大的经济损失。本研究从疑似牛病毒性腹泻-黏膜病的粪便中分离得到一株病毒能使MDBK细胞产生细胞病变,经RT-PCR检测及扩增产物测序进一步证实,该病毒为牛病毒性腹泻-黏膜病病毒,TCID50测定该病毒的滴度为107.15TCID50/ml。该病毒株的分离鉴定为牛病毒性腹泻病毒疫苗的研制奠定了基础。

Bovine viral diarrhea virus （BVDV） is the causative agent of bovine viral diarrhoea, and is u- biquitous in cattle. It is very complex and presents a wide range of clinical manifestations, which causes large economic loss to cattle industry around the world. In this study, a virus strain causing cytopatbic effect of ma- din- darby bovinekidney （MDBK） was isolated from PCR and sequencing analysis. The virus titer was 107 rus strain laid foundation for exploring its vaccine. the bovine excrement and identified as BVDV by RT- ^7.15 TCID50/ml. The isolation and identification of this virus strain laid foundation for exploring its vaccine.