摘要
锌指蛋白核酸酶(Zinc Finger Nuclease,ZFN)是一种人工合成酶,含有锌指蛋白和FokⅠ核酸内切酶的剪切结构域,因其能特异性识别并切割DNA序列及其可设计性而被用基因定点突变和外源基因定点整合。这项技术已应用于转基因动物研究中。本文综述了锌指核酸酶技术在转基因研究中的应用进展,并对锌指核酸酶技术的应用前景进行了展望。
Zinc finger nuclease （ZFN） is a kind of artificial synthesis containing zinc finger protein and the shear structure domain of Fok I endonuclease. Because it can specifically identify and cleave DNA se- quences and easily be designed, it is used for the site - directed genetic mutations and integration of exogenous genes. This technology has been applied to the research of transgenic animals. In this paper, the research pro- gress of zinc finger nuclease technology and its application were reviewed, and its application prospect was also forecasted.
出处
《山东农业科学》
2013年第2期135-138,共4页
Shandong Agricultural Sciences
基金
泰山学者海外特聘专家(何洪彬)
国家奶牛产业技术体系建设专项经费(何洪彬)
山东省科技攻关课题(2009GG20002032)
国家转基因重大专项(2009ZX08007-006B
2011ZX08007-002
2011ZX08008-004)
济南市高校院所自主创新计划(201004027)
关键词
锌指蛋白
锌指核酸酶
基因打靶
同源重组
转基因动物
Zinc finger protein
Zinc finger nuclease
Gene targeting
Homologous recombination
Transgenic animal
