固定化球形红细菌生物合成银纳米材料及其抑菌性能研究

Biological Synthesis of Silver Nanomaterials Using Immobilized Rhodobacter Sphaeroides and Their Antimicrobial Activities

采用固定化球形红细菌生物合成银纳米材料,对所制备的银纳米材料分别用紫外吸收光谱、X射线衍射、低分辨和高分辨透射电镜等做了详细的表征。结果表明,制备的银纳米材料为立方闪锌矿结构,呈圆柱状,尺寸大小变化范围为10～50 nm。体外抑菌结果显示,对于大肠杆菌和金黄色葡萄球菌,其最小抑菌浓度为10 mg/L,最小杀菌浓度为80 mg/L。

Silver nanomaterials were successfully biosynthesized by immobilized Rhodobacter sphaeroides. The silver nanomaterials were characterized by means of UV-Vis optical absorption, X-ray diffraction （XRD）, transmission electron microscopy （TEM）, high-resolution transmission electron microscopy （HRTEM）, energy dispersive analyses of X-rays （EDX）. The silver nanomaterials are cubic zinc blende strucaure and circular columns-shaped, and the nanoparticles are found to be polydisperse in the size range 10-50 nm. In addition, the silver nanomaterials showed high antimicrobial activities against E. coli. and S. aureus. The results showed that the minimum inhibitory concentration （MIC） and the minimum bactericidal concentration （MBC） of the silver nanoparticles are 10 mg/L and 80 mg/L, respectively.