摘要
目的探讨COX-2在对乙酰氨基酚致大鼠药物性肝损伤中的作用。方法选择40只SD大鼠随机分成对照组、塞来昔布对照组(A组)、对乙酰氨基酚组(B组)、对乙酰氨基酚+塞来昔布组(C组),每组10只。单次给药,24小时后采集血液测定ALT、AST、ALP、TBA等生化指标,之后处死大鼠,取其肝脏组织行病理学检查,应用免疫组织化学染色方法检测COX-2的表达。结果 B组肝损伤明显,C组肝损伤较B组严重。对照组大鼠肝脏组织未见COX-2的阳性表达;B组有7例COX-2表达呈阳性,阳性率为70.0%;C组仅有1例COX-2表达呈阳性,阳性率为11.1%。结论 COX-2在对乙酰氨基酚所致大鼠急性肝损伤肝组织中表达明显升高,阻断COX-2后,肝损伤加重。COX-2表达增高可能是机体对抗对乙酰氨基酚所致大鼠急性肝损伤的一种保护性机制。
Objective To investigate the role of COX-2 in acetaminophen-induced liver injury in rats. Methods Total of 40 male SD rats were randomly divided into 4 groups： control group, celecoxib treated group （group A）, acetaminophen treated group （group B）, and acetaminophen combined with celecoxib treated group （group C）. Each group was comprised of 10 rats and each rat was given a single dose of appropriate drug. After 24 hours, blood was collected to perform a serum biochemical test of liver function, and liver tissue was harvested for pathological examination and COX-2 expression. Results There was obvious liver damage in group B, and liver injury of group C was aggravated by COX-2 inhibition than group B. There was no expression of COX-2 in hepatic tissue in control group. Positive COX-2 expression were observed in 7 rats of group B, with the rate of 70.0% and only one rat in group C, with the rate of 11.1%. Conclusions Intensity of COX-2 expression increased in group B and liver damage were aggravated after COX-2 blocking-up in the presence of COX-2-selective inhibitors. Induction of COX-2 expression may be a protective mechanism against acute liver injury induced by acetaminophen.
出处
《中国肝脏病杂志（电子版）》
CAS
2011年第4期5-8,共4页
Chinese Journal of Liver Diseases：Electronic Version
基金
广东省自然科学基金(2009B030801142)
