肾透明细胞癌组织中CMTM家族的表达及意义

Expression of CMTM3 and CMTM5 in clear cell renal cell carcinoma and its significance

目的探讨CMTM家族（CKLF—like MARVEL transmembrane domain—containing family）成员CMTM3和CMTM5在肾透明细胞癌（clear cell renal cell carcinoma，ccRCC）组织中的表达及意义。方法ccRCC标本75例，男50例，女25例。年龄（59±10）岁。2009AJCC分期I期34例，Ⅱ期23例，Ⅲ期14例，Ⅳ期4例。Fuhrman核分级I级3例，I～Ⅱ级1例，Ⅱ级35例，Ⅱ～Ⅲ级10例，Ⅲ级18例，Ⅲ～Ⅳ级3例，Ⅳ级5例。应用免疫组织化学方法结合组织微阵列技术检测ccRCC组织及相应正常肾组织中CMTM3、CMTM5蛋白的表达情况，综合临床资料进行分析。结果正常肾组织中CMTM3、CMTM5蛋白阳性表达率分别为98．7％和97．3％，ccRCC组织的阳性表达率分别为44．0％和68．O％，组间差异有统计学意义（P〈0．05）。ccRCC组织中CMTM3、CMTM5蛋白表达程度与性别、年龄、分期、病理分级无明显相关（P〉0．05）。结论CMTM3和CMTM5是ccRCC抑癌基因，其突变或异常甲基化可能在肾癌的发生过程中起重要作用，有望成为肾癌的早期诊断指标。

Objective To investigate the expression of CKLF-like MARVEL transmembrane （CMTM） domain-containing family in clear cell renal cell carcinoma （ccRCC） and its significance. Methods Seventy-five samples of ceRCC were collected, including 50 males and 25 females, mean age （59 ＋ 10） years. There were 34 cases in clinical stage I, 23 cases in stage II, 14 cases in stage III and 4 cases in stage IV. The pathological differentiation was 3 cases of grade I, 1 case of grade I - II, 35 cases of grade II, 10 cases of grade II -III, 18 cases of grade lII, 3 cases of grade III- IV and 5 cases of grade IV. The expression of CMTM3 and CMTM5 proteins in 75 cases of ccRCC and corresponding adjacent normal kidney tissues was detected by tissue microarray and immunohistochemistry. Results The positive expres- sion rate of CMTM3 and CMTM5 was 98.7% , 97.3% in the adjacent normal kidney tissues, and 44.0% , 68.0% in ccRCC tissues （P 〈 0.05）. The expression of CMTM3 and CMTM5 had no correlation with the gender, age, clinical staging and pathological differentiation （P 〉 0. 05）. Conclusion CMTM3 and CMTM5 could be ccRCC suppressor genes, and their mutation or methylation might be an early event in the carcinogenesis of ccRCC, thus promise them to be potential biomarkers in early diagnosis.