阻断PD-L1通路对膀胱肿瘤患者外周血树突状细胞免疫功能的影响

Immunological function variation of peripheral dendritic cell from bladder cancer patients after bloc- king PD-L1 pathway

目的研究阻断膀胱肿瘤患者外周血树突状细胞（dendriticcell，DC）PD—L1通路对其免疫功能的影响。方法通过rhGM．CSF、rhIL-4在体外诱导膀胱肿瘤患者和正常人外周血DC，PD—Ll单克隆抗体处理后，流式细胞仪检测CD1a HLA和CD83的表达；噻唑盐比色法和酶联免疫吸附法检测其刺激淋巴细胞增殖和分泌IL-10和IL—12能力的变化。结果阻断PD—L1通路对DC成熟无明显影响，但可以使膀胱肿瘤患者DC刺激淋巴细胞增殖能力增强（4．004-1．28和1．494-0．45），并增强IL-12[（108．30±21．89）ng／L和（37．17±14．89）ng／L]、减少IL-10[（108．904-21．77）ng／L和（214．994-54．99）ng／L]的分泌（P〈0．05）。结论阻断PD—L1通路可使膀胱肿瘤患者外周血DC的免疫活性增强，提示可尝试通过PD—L1通路调节膀胱肿瘤患者的免疫功能，为防治膀胱肿瘤复发提供新途径。

Objective To investigate the immunological function variation of peripheral dendritic cell （DC） from bladder cancer patients after blocking PD-L1 pathway. Methods DC from normal control and patient with bladder cancer were cultured with rhGM-CSF, rhIL-4 in vitro and treated with PD-L1 mono- clonal antibody. The expression of CDla, HLA and CD83 were examined by flow cell meter. The effect of DC induced lymphatic cellular proliferation and its capability of secreting IL-10, IL-12 were determined by MTT and ELISA. Results Blocking PD-LI pathway did not infuluence the maturation of DC. But the DCs from bladder cancer patient signifcantly boosted the lymphatic cellular proliferating （4.00 ± 1.28 versus 1.49 ± 0.45） and IL-12 secretion capability （108.30 ± 21.89 versus 37. 17 ± 14.89 ng/L） , and yet it also de- creased the secretion of IL-10 （ 108.90 ± 21.77 versus 14.99 ± 54.99 ng/L） after blocking PD-L1 pathway （P 〈 0.05）. Conclusion Blocking PD-LI pathway on the DC from bladder cancer patient may improve its anticancer immunological function.