摘要
目的 探讨Livin表达影响水疱口炎病毒（VSV）对肺癌A549细胞的促凋亡效应。方法 利用RNA干扰技术下调Livin在肿瘤细胞中的表达，通过Tunel染色观察VSV对肺癌A549细胞的凋亡效应，Western blot法检测Livin蛋白的表达，荧光定量法检测Caspase-3的活性。结果 VSV（感染复数＝ 0.1 pfu／细胞）对A549细胞有促凋亡作用；抑制Livin的表达增强了VSV对A549细胞的促凋亡作用，增强了Caspase-3的活性。结论 Livin表达沉默后可能通过影响Caspase-3活性而增强了VSV对A549细胞的促凋亡效应。
Objective To investigate the effect of Livin expression on VSV-induced apoptsis of A549 cells. Methods The expression of Livin of A549 cells was inhibited by RNA interference. VSV-induced apoptosis of A549 cells was observed by Tunel assay. Protein Level of livin was detected by Western blot. Caspase-3 activity was detected by the fluorescence-based quantitative method. Results Livin downregulation VSV-induced apoptosis of A549 cells. Inhibited the expression of Livin of A549 cells had increased Caspase-3 activity. Conclusion The effect of Livin on VSV-induced apoptotic of A549 cells could be increased by RNA interference.
出处
《肿瘤研究与临床》
CAS
2013年第1期5-7,共3页
Cancer Research and Clinic
