摘要
目的探讨间歇缺氧对大鼠血清以及心肌中NF—kBp65以及MCP-1含量的影响。方法清洁SD大鼠30只,随机分成3组(每组10只):正常对照组、间歇缺氧组、持续缺氧组,普通饲料饲养。正常对照组:常压、常氧下饲养;间歇低氧组:每90s-循环,氧舱内低氧浓度维持在6%~8%,持续10s以上;持续低氧组:氧舱内氧浓度维持在8%~11%。每天早上9:00动物入舱,至下午5:00试验结束,每天持续8h,连续35d。然后经右颈动脉抽血,处死大鼠分离其心脏,采用酶联免疫吸附法(ELISA法)测定血清中NF—kBp65以及MCP~1含量,采用免疫印迹法测定大鼠心肌中NF—kBp65以及MCP-1的含量。结果与正常对照组相比,在间歇缺氧组以及持续缺氧组中大鼠血清以及心肌中NF—kBp65以及MCP-1的含量明显增加。结论间歇缺氧可导致NF—kB的激活,从而导致大鼠血清以及心肌中NF—kBp65以及MCP-1的表达增加。
Objective To investigate the expression of NF-kBp65 and MCP-1 in serum and heart muscle in intermittent hypoxia rats. Methods There are thirty SD rats were randomly assigned into three groups: control group, intermittent hypoxia group and continuing hypoxia group, which were exposed to air, intermittent hypoxia and continuing hypoxia respectively 8h/d for 35d.Then the blood and heart samples were collected, and the NF-kBp65 and MCP-1 levels in serum and in heart tissue were measured with ELISA and Western blot respectively. Results Compared to the control group the contents of NF-kBp65 and MCP-1 in serum and heart were increased both in intermittent hypoxia group and continuing hypoxia group. Conclusion NF-kBp65 and MCP-1 in heart and blood of intermittent hypoxia rats, which may be associated with the activation of NF-kB by hypoxia.
出处
《浙江医学》
CAS
2013年第1期14-15,19,共3页
Zhejiang Medical Journal
基金
温州市科技局资助项目(Y20090125)