miR-139—3p在胃癌组织中的表达及其对细胞增殖和凋亡的作用

Effect of miR-139-3p on cell proliferation and apoptosis in gastric cancer CHEN Legao,

目的研究miR-139—3p在胃癌组织和癌旁组织中的表达水平及其对AGS和SGC-7901胃癌细胞株的增殖和凋亡的作用。方法采用qRT—PCR检测38例患者胃癌组织与癌旁组织中miR-139—3p的表达情况，并分析miR-139—3p的表达与胃癌临床病理特征之间的关系；利用miR-139—3p抑制剂下调胃癌细胞株中miR-139—3p的表达，MTT法检测对AGS和SGC-7901细胞增殖的影响，流式细胞仪检测细胞凋亡情况。结果miR-139—3p在胃癌组织中表达高于癌旁组织（P〈0.01），其表达增高与肿瘤大小和部位相关（均P〈0．05）；下调miR-139—3p的表达能够抑制胃癌细胞的增殖、促进细胞凋亡（均P〈005）。结论miR-139—3p在胃癌组织中表达增高，可能参与胃癌的发生、发展过程。

Objective To investigate the effect of miR-139-3p on proliferation and apoptosis in gastric cancer. Methods The expression of miR-139-3p in gastric cancer and pericancerous tissues was examined by Real-time PCR. The correlation between miR-139-3p expression and clinicopathologic features of gastric cancer was analyzed. The cultured gastric cancer AGS and SGC-7901 cells were treated with miR-139-3p Inhibitor; the cell proliferation was detected by M]q- assay and apopto- sis was analyzed by flow cytometry. Results The expression of miR-139-3p increased in gastric cancer compared with peri- cancerous tissues （P〈0.05）. Expression of miR-139-3p was associated with tumor size （P〈0.05） and location （P〈0.05）. Down-regulation of miR-139-3p inhibited proliferation and promoted apoptosis of gastric cancer AGS and SGC-7901 cells. Conclusion miR-139-3p is up-regulated in gastric cancer, which might be involved in the development of gastric cancer.