大肠癌组织中Ezrin和PTEN蛋白的表达及相关性研究

Expression of Ezrin and PTEN proteins in colorectal carcinoma

目的探讨大肠癌组织中Ezrin和PTEN蛋白表达的意义。方法采用免疫组化SP法检测80例大肠癌组织中Ezrin和PTEN蛋白的表达，并分析其与临床和病理特点的关系。结果大肠癌组织中Ezrin蛋白表达阳性率为77．5％（62／80），显著高于正常大肠黏膜组织（P〈0．01）；PTEN蛋白表达的阳性率为46．3％（37／80），显著低于正常大肠黏膜组织（P〈0．01）；Ezrin蛋白高表达PTEN蛋白低表达与大肠癌Dukes分期、浆膜浸润、淋巴结转移有关（均P〈001），而与年龄和组织学类型无关（均，’〉O．05）；Ezrin和PTEN蛋白表达呈负相关（P〈0．01）。结论Ezrin和PTEN蛋白表达与大肠癌临床病理特征和生物学行为有密切关系，联合检测Ezrin和PTEN蛋白的表达，可用于评估大肠癌侵袭转移能力，对大肠癌的预后判断具有一定意义。

Objective To investigate the expression of Ezrin and PTEN proteins in colorectal adenocarcinoma and its sig- nificance. Methods The expression of Ezrin and PTEN proteins was examined immunohistochemically with SP method in 80 cas- es of colorectal adenocarcinoma, and the relationships of the expression with clinicopathological characteristics of tumor was an- alyzed. Results The positive rates of Ezrin and PTEN protein expression in 80 cases of colorectal adenocarcinoma were 77.5% （62/80） and 46.3%（37/80） respectively. They were not correlated with age or histological type（P 〉0.05）. The high level of Ezrin pro- tein expression and the low level of PTEN protein expression were correlated significantly with the Dukes＇ stage, serosa infiltration, lymph node metastasis of colorectal adenocarcinoma （P〈0.01）. The expressions of Ezrin protein was negatively correlated with that of PTEN protein（P〈0.01）. Conclusion The expressions of Ezrin and PTEN are correlated with the clinicopathological charac- teristics and biologic behaviors of colorectal adenocarcinoma. The combined detection of Ezrin and PTEN proteins may be helpful in evaluation of prognosis and aggressive potential of colorectal adenocarcinoma.