共轭亚油酸对环孢酶素A注射仔猪细胞免疫反应的影响

Effects of dietary conjugated linoleic acid on cell immunity response of piglets after CsA injection

为研究日粮添加共轭亚油酸(Conjugated linoleic acid,CLA)对细胞免疫抑制剂环孢酶素A(CiclosporinA,CsA)注射仔猪细胞免疫反应的影响,采用2×2试验设计即日粮类型(2%CLA或2%大豆油)和细胞免疫抑制处理(注射CsA或生理盐水),48头21d断奶仔猪随机分为4个处理组,试验期为42d。注射CsA显著提高仔猪的料重比(P<0.05),而日粮添加CLA显著降低仔猪的料重比(P<0.05),且日粮CLA阻止因CsA注射引起的仔猪料重比提高(P<0.05)。CsA注射极显著降低胸腺CD4+和CD8+T淋巴细胞百分数(P<0.01)。日粮CLA显著提高胸腺CD4+CD8+双阳性和CD8+单阳性T淋巴细胞百分数(P<0.05),且阻止注射CsA引起的仔猪胸腺CD4+单阳性、CD8+单阳性T淋巴细胞百分数的降低(P<0.01)。CsA注射严重耗竭仔猪外周血CD3+、CD4+和CD8+T淋巴细胞(P<0.01)。无论注射CsA与否,日粮添加CLA均提高仔猪外周血CD8+T淋巴细胞数和IL-2诱生活性(P<0.05),显著缓解注射CsA引起仔猪外周血CD3+、CD4+和CD8+T淋巴细胞数的减少和IL-2诱生活性的降低(P<0.01)。在无CsA注射的情况下,日粮CLA对仔猪外周血淋巴细胞转化率没有显著影响(P>0.05),但日粮CLA显著抑制因CsA注射引起的仔猪外周血淋巴细胞转化率降低(P<0.05)。日粮添加CLA能显著缓解因CsA注射引起的仔猪细胞免疫抑制。

The present experiment was conducted to investigate effects of conjugated linoleic acid （CLA） on cell immunity response of piglets after Cyclosporin A （CsA） injection. The experiment design was a 2 × 2 factrial design,and the main factors consisted of diets （2% CLA or 2% soybean oil）and immunosuppression treatment（CsA or saline injection）. Fourty-eight weaned piglets at 21days were randomly allocated into four experimental groups. The experimental period was 42 days. CsA injection significantly increased Feed/Gain （F：G） of piglets（P〈0.05）, however dietary CLA significantly decreased F： G of piglets（P〈0.05）, moreover CLA diets ameliorated the increase of F：G resulted from CsA injection （ P〈0.01 ）. CsA-treatment significantly decreased the percentage of CD4＋ and CD8＊ T lymphocytes in thymus （P〈0.01）. 2% CLA diets resulted in higher percentage of CD4＋ CD8＋ doublepositive and CD8＋ siglepositive T lymphocytes in thymus（P〈0.05）, and decrement of CD4＋ and CD8＋ T lymphocytes of thymus resulted from CsA injection was lesser in piglets fed the CLA diet compared with those fed basal diets（ P〈0.01 ）. CsA- treatment significantly depleted the number of CD3＋ , CD4＋ and CD8＋ T lymphocytes of peripheral blood. Whether with CsA injection or not,dietary CLA significantly increased CD8＋ T lymphocyte subpopulations of peripheral blood and IL- 2 production（P〈0.05）. The decrement of CD3＋ ,CD4＋ and CD8＋ T lymphocyte of peripheral blood and IL-2 productionwas notably suppressed in piglets fed the CLA diet（ P〈0.01 ）. Without CsA injection treatment, dietary CLA didn＇t influence lymphocyte proliferation of peripheral blood（P〈0.05）, but notably suppressed the decrease of lymphocyte proliferation resulted from CsA injection （P 〈 0. 05）. Dietary CLA effectively ameliorated cell immunosuppression resulted from CsA injection.