新疆沙枣中原花青素的提取工艺研究

Study on the extraction process of proanthocyanidin from Xinjiang Elaeagnus angustigolia L.Jujubes

目的:对新疆沙枣中原花青素的提取工艺进行研究,方法:采用正丁醇-盐酸比色法检测原花青素含量。在单因素基础上,采用正交L9(34)设计,考察乙醇浓度、提取时间、pH、料液比、提取温度对沙枣中原花青素含量的影响,并用AB-8型大孔树脂对其进行柱层析分离及纯化。结论:试验结果表明在乙醇浓度70%,提取时间120min、pH为5、料液比为1∶9、提取温度80℃的条件下提取效果最好。在最佳提取工艺条件下,AB-8型大孔树脂对沙枣果肉中的原花青素的动态吸附率为73.9%,用50%乙醇洗脱,其解析率达87.38%,具有较好的纯化效果。

objective The extraction process of proanthocyanidi from Elaeagnus angustigolia L. Jujubes was studied. methods the proanthncyanidin content was determined in butanol -HCL method. Based on single factor test, the etha- nol eoncentration, extraction time, pH, extraction temperature, material -liquid ratio were selected as factors in a L9 （34 ） orthogonal experiment. Conclusion The optimal extraction conditions were ： ethanol concentration of 70% , ex- traction temperature of 120rain, pH of 5, extraction temperature 80℃, material - liquid ratio 1 ： 9. AB - 8 macro- porous resin was preferable for Separating Elaeagnus angustigolia L. Jujubes with the dynamic absorption and desorp- tion ratio of 73.9% and 87.38% respectively.