姜黄素对糖尿病大鼠氧化应激的影响

Effect of curcumin on oxidative stress in diabetic rats

目的:观察姜黄素对糖尿病大鼠氧化应激的影响。方法:用链脲佐菌素(streptozotocin,STZ)腹腔注射建立糖尿病大鼠模型。将大鼠随机分为正常对照组、糖尿病组、姜黄素灌胃组。成模12周后,检测各组大鼠血糖、血肌酐、24h尿蛋白水平,肾组织中丙二醛(MDA)、过氧化氢酶(CAT)含量及总超氧化物歧化酶(T-SOD)活性,常规制作石蜡切片,Masson染色观察肾脏病理形态改变。结果:与糖尿病组相比,姜黄素治疗组大鼠血糖、血肌酐、24h尿蛋白及肾脏MDA含量明显下降,而肾脏抗氧化酶T-SOD活性及CAT含量则显著上升,肾脏形态明显改善。结论:姜黄素能够提高肾功能及肾脏抗氧化能力,具有糖尿病大鼠肾脏保护作用。

Objective： To evaluate the effect of Curcumin on oxidative stress in diabetic rats. Methods： Diabetic rat models were induced by the intraperitoneal injection of streptozotocin （STZ）. Method： Thirty Sprague Dawley （SD） rats （male） were divided into three groups： the normal rats, the diabetic rats, the diabetic rats treated with Curcumin （200mg/kg/d）. After 12 weeks, the levels of blood glucose, serum creatinine, 24h urinary protein, the content of malondialdehyde （MDA）, catalase （CAT） and total superoxide dismutase （T- SOD） activites in the renal tissue were measured. Paraffin sections were made using routine method, stained with Massonand observed under light micro- scope to observe the histopathological changes. Results： Compared with the diabetic model group, the levels of blood glucose, serum creatinine, 24h urinary protein and MDA, CAT in the renal tissue were significantly decreased in Cur- cumin treated group and the activity of T - SOD in the renal tissue was increased. In addition, the renal pathologic changes in Curcumin treated group were also improved. Conclusions： Curcumin can increase the renal function and an- tioxidant capacity. Therefore, it has protective effect on kidney of diabetic rats.