改良固相萃取高效液相色谱法测定人血清中万古霉素浓度

Determination of the Concentration of Vancomycin in Human Serum by Modified Solid Phase Extraction and HPLC

目的:建立高效液相色谱法测定人血清中万古霉素浓度的方法。方法:以HypersilC18柱(4.6mm×200mm,10μm)为色谱柱,流动相为乙腈-磷酸二氢钾缓冲液(9∶91),流速为0.8mL/min,检测波长236nm。血清样品采用固相萃取柱,以含20%乙腈的磷酸二氢钾溶液为洗脱剂,收集洗脱液直接进样测定。结果:万古霉素血清样品线性范围为0.8～120mg/L,低、中、高3种不同浓度(4.33、19.40、77.60mg/L)回收率分别为100.23%、102.11%、95.68%,日内、日间RSD均小于5%。结论:该方法准确性好、灵敏度高、操作简便,可满足临床万古霉素血药浓度检测的需要。

Objective ： To establish a high performance liquid chromatography method for the determination of the concentration of vancomycin in human serum. Methods：The analysis was conducted with Hypersil C18 column （4.6 mm 200 mm, 10 μm）. Acetonitrile-potassium dihydrogen phosphate （9：91） was served as mobile phase, the flow rate was 0.8 mL/min, and the detecting wavelength was at 236 nm. Serum sample was applied to solid phase extraction column. Taking the potassium dihydrogen phosphate solution containing 20% acetonitrile as eluent, and collecting the eluent which was determined by direct injection. Results：The linear range of vancomycin in serum was 0.8 ~ 120 mg/L. The recovery rate of low, moderate and high concentrations （4.33,19.40,77.60 rag/L） was 100.23%, 102.11%, 95.68% respectively. Both intra-day and inter-day RSDs were less than 5%. Conclusion：This method is accurate, sensitive, and simple. It is suitable for the determination of the concentration of vancomycin in human serum.