响应面设计法优化不同基源贝母中总生物碱的提取工艺

Extraction and Determination of Total Alkaloids from Bulbus Fritillariae

为获得贝母总生物碱的最佳提取工艺参数,以川贝母作为工艺参数研究对象,运用响应面试验设计探讨以氯仿和甲醇体积比、液料比、提取时间作为提取工艺考察因素,以总生物碱为考察指标,用比色法测定不同基源贝母中总生物碱的含量,优化出贝母的最佳提取工艺,并比较不同基源贝母在该最佳工艺条件下总碱的含量差异。结果贝母总生物碱的最佳提取工艺参数为:氯仿:甲醇4.2:1(V/V)、液料比30:1、提取4h,此条件下,四川松潘川贝母的总生物碱含量2.84mg/g。不同基源贝母中,湖北贝母总生物碱的含量最高,达5.379mg/g。本方法结果可靠、操作简便,优化后的最佳提取条件适合贝母总碱的批量提取。

This study was carried out to establish optimum conditions for the extraction of total alkaloids from Bulbus Fritillariae and to compare the extraction efficiency of total alkaloids from Bulbus Fritillariae from different species and growing areas under optimum extraction conditions. Response surface methodology was used to optimize the extraction of total alkaloids from Bulbus Fritillariae cirrhosae. The optimal extraction conditions were found to be 4.2：1 （V/V）, 30：1 and 4 h for CHC1JCH3OH ratio, solid-to-solvent ratio and extraction time, respectively. The extraction efficiency of total alkaloids from Bulbus Fritillariae cirrhosae under these conditions was 2.84 mg/g compared to 5.379 mg/g from Bulbus Fritillariae hupehensis, highest among the five Fritillaria species investigated. The optimized extraction method was simple, reliable and allowed large-scale extraction of total alkaloids from Bulbus Fritillariae.