姜黄素对大鼠子宫内膜异位症激素及受体的影响

Influence of crucumin on the expression of hormones and their receptors in rats with endometriosis

目的:探讨姜黄素(crucumin)治疗大鼠子宫内膜异位症的治疗机制。方法:采用雌性SD清洁级大鼠建立模型子宫内膜异位症模型,分为空白对照组、模型组、孕三烯酮组、姜黄素组。给药3周后,酶联免疫反应法(ELISA)测血清雌二醇(E2)、孕酮(P)的含量,SP法检测异位子宫内膜组织雌激素受体α(ERα)、雌激素受体β(ERβ)、孕激素受体(PR)的表达。结果:孕三烯酮组、姜黄素组血清E2、P含量和异位组织ERα、ERβ、PR的表达与模型组比较差异有统计学意义(P<0.05),孕三烯酮组、姜黄素组组间无明显差异(P>0.05)。结论:姜黄素能降低血清E2、大鼠子宫内膜异位组织ERα、ERβ表达,提高血清P含量与异位组织PR的表达,抑制模型大鼠异位内膜的生长,实现治疗目的。

OBJECTIVE To investigate the mechanism of action of crucumin in rats, which had some positive treatment results to endometriosis in clinic. METHODS The endometriosis model was established in clean female SD rats. After 3 weeks, the rats were divided into 4 groups： sham operated rats as control group, model group, gestrinone group and crucumin group. After 3 weeks of treatment, the expression of ERa, ER~ and PR was determined by immunostaining. RESULTS In the gestri- none group and crucumin group, the expression of ERa, ER^I in the ectopic tissue was signifidant difference compared with model group（P^0. 05）. However, there was no obvious difference between the gestrinone group and crucumin group （P〈0. 05）. Both gestrinone group and crucumin group, the expression of PR ectopic tissue was significant difference with the model group （P〈0. 05） ,crucumin group higher than gestrinone group, the difference had statistical significance （P〈0. 05）. CONCLUSION Crucumin can inhibit the ERa, ERβ and improve PR expression in the ectopic endometria of rats and can inhibit models rats ectopic endometrial growth, realized the objective of treatment.