金胆片对大鼠肝内胆汁淤积模型的预防作用

Study on the preventive effect of Jindan tablets on intrahepatic cholestasis in rats

目的:探讨金胆片对α-萘异硫氰酸酯(ANIT)诱导大鼠肝内胆汁淤积的预防作用。方法:Wister大鼠随机分为正常对照组、模型对照组、阳性对照组、金胆片低、高剂量组。灌胃给予金胆片4 d后,采用ANIT诱导大鼠肝内胆汁淤积模型。造模后4 d测定大鼠胆汁流量和流速,以及血清总胆红素(TBIL)、直接胆红素(DBIL)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和总胆汁酸(TBA)等生化指标的含量。取大鼠肝组织进行切片,HE染色,显微观察病理学改变。结果:金胆片可以显著增加肝内胆汁淤积大鼠的胆汁流量和流速,降低血清TBIL、DBIL、ALT、AST和TBA水平(P<0.05);并可显著缓解胆汁淤积状态下的肝细胞损伤。结论:金胆片对肝内胆汁淤积具有一定的预防作用,具有较好的临床价值。

OBJECTIVE To investigate the preventive effect of Jindan tablets on intrahepatic cholestasis induced by alphanaphthylisothiocyanate （ANIT） in rats. METHODS Wistar rats were randomly divided into 5 groups： normal control group, model group, positive control group, low-dose Jindan tablets group and high-dose Jindan tablets group. Cholestatic model of rats was induced by ANIT 4 days after oral administration of Jindan tablets. The flow and speed of bile were determined 4 days after the model was established, as well as the biochemical indicators, such as TBIL, DBIL, ALT, AST and TBA. Liver tis sues of rats were taken for histopathologic analysis. RESULTS Jindan tablets increased the flow and speed of bile in rats with intrahepatic cholestasis, and significantly decreased the serum levels of TBIL, DBIL, ALT, AST and TBA （P〈0. 05）. Histopathology of livers showed the protective effect of Jindan tablets on injury of hepatocytes in condition of cholestasis. CONCLUSION J indan tablets have a significant effect on improving intrahepatic cholestasis, and have good prospects in clinic.