XPD Lys751Gln基因多态性与非霍奇金淋巴瘤化疗后生存期及白细胞抑制的关系

Association Between the Polymorphism of XPD Lys751Gln and the Survival as Well as the Leukocyte Inhibition After Chemotherapy in Patients with Non-Hodgkin's Lymphoma

[目的]探讨核苷酸切除修复基因XPD Lys751Gln基因多态性与非霍奇金淋巴瘤(NHL)化疗后疗效﹑2年生存期及化疗相关的白细胞抑制程度的相关性。[方法]在广西人群中,收集87例NHL患者的外周血样本,采用聚合酶链反应—限制性片段长度多态技术检测XPD Lys751Gln位点的基因多态性,并应用非条件Logistic回归法进行数据分析,分析各基因型与NHL化疗后疗效﹑生存期及化疗相关白细胞抑制程度的相关性。[结果]XPD Lys751Gln(T>G)多态性位点在87例NHL病例中,TT基因型的频率为81.6%(71例),TG基因型为18.4%(16例),未发现GG基因型。XPD Lys751Gln的TT基因型及TG基因型的频率在NHL患者化疗有效病例组与无效组之间,总生存期≥2年病例组与<2年组之间及NHL患者化疗相关白细胞抑制不同程度分组间的分布均没有统计学差异(均为P>0.05)。[结论]在中国广西的人群中,XPD基因的Lys751Gln多态性与NHL患者化疗后疗效、2年生存期及化疗后相关白细胞抑制程度均无显著性关联。

[Purpose] To explore the relationship between the single nucleotide polymorphism of XPD Lys751Gln and the response to chemotherapy as well as the 2 years survival and the degree of chemotherapy-related leukocyte inhibition in patients with non-Hodgkin＇s lymphoma（NHL）. [Methods] Eighty-seven NHL patients, as residents of Guangxi Zhuang autonomous region of China, were enrolled into the study. Blood sample was obtained at diagnosis. The single nucleotide polymorphism of XPD Lys751G1n were detected using PCR-restriction fragment length polymorphism assay. Based on uncon- ditional Logistic regression analysis,the association between different gene types and the response to chemotherapy,survival as well as the chemotherapy-related leukocyte inhibition in patients with NHL was analyzed. [Results] In the XPD Lys751Gln（T〉G） polymorphism of the eighty-seven NHL patients, frequency of TI＂ genotype was 81.6% （71 cases）; TG, 18.4% （16 cases） and no GG genotype. The fre- quencies of TI and TG genotypes showed no significant difference between the response group and non-response group,also no significant difference between the group of surviving over 2 years and group of surviving less than 2 years, or among groups with different degree of leukocyte inhibition after chemotherapy in patients with NHL （P〉0.05）. [Conclusion] There is no association between the poly- morphism of XPD Lys751Gln and response to chemotherapy as well as the two-years survival and the degree of leukocyte inhibition after chemotherapy in patients with NHL in Guangxi.