不同瘤胃乙、丙酸比例对绵羊丙酸糖异生和葡萄糖周转速度的影响

INFLUENCE OF RUMINAL ACETATE TO PROPIONATE RATIO ON GLUCONEOGENESIS OF PROPIONATE AND GLUCOSE TURNOVER IN SHEEP

本项试验使用了 14C标记的丙酸和葡萄糖 ;用同位素示踪技术研究了全营养灌注绵羊改变瘤胃灌注混合挥发性脂肪酸中乙/丙酸比例后 ,丙酸糖异生代谢和体内葡萄糖周转速度的变化。结果表明 ,灌注乙/丙酸比例为75 :15的混合挥发性脂肪酸时 ,葡萄糖碳原子中来自丙酸的碳原子比例(P<0.01)和体内葡萄糖周转速度(P<0.05)均高于灌注其它乙/丙酸比例的混合挥发性脂肪酸。

C-labelled propionate and -14C-glucose were used as a tracer to measure the percentage of glucose carbon from propionate and glucose turnover of the sheep sustained by intragastric nutrition when volatile fatty acid mixtures with three acetate to propionate ratios were infused.The three acetate to propionate ratios were 75:15 (VFA1),65:25(VFA2) and 45:45(VFA3),respectively.The percentages of glucose carbon from propionate in blood samples drained from jungular vein of the sheep, when different VFA mixtures were infused, were 29.5±1.05(VFA1),24.35±2.17(VFA2)and 25.21±1.15(VFA3) respectively,with the highest percentage for VFA1 (P<0.01).Glucose turnovers of the sheep,when VFA1 or VFA2 were infused ,were (5.16±0.16) g/h and(4.69±0.08 )g/h respectively,which indicated that when VFA1 was infused,glucose turnover was increased(P<0.01).