血清蛋白指纹图谱在食管癌中的应用

Application of serum surface-enhanced laser desorption/ionization proteomic patterns in esophageal carcinoma

目的采用蛋白质芯片技术分析食管癌患者血清蛋白质谱的差异，探讨其临床应用价值。方法采用表面增强激光解析电离飞行时间质谱（SELDI．TOF-MS）技术，使用弱阳离子交换（WCX-2）蛋白芯片分别检测食管癌患者放疗前后、接受调强放疗和普通放疗、转移和非转移患者的血清蛋白指纹图谱，采用蛋白质芯片阅读机读取数据，CiphergenProteinChip3．0分析软件采集数据，读取峰信息，进行统计学分析。结果在放疗前后食管癌患者血清中，发现质荷比为3884．213、1699．600、1371．126、8454．341、1141．881、1473．377、2086．908、2785．016的8个蛋白峰差异具有统计学意义；在接受不同放疗方式食管癌患者血清中，发现质荷比为3470．098、2011．529、3395．789、2639．055、1511．632、11488．520、9546．555、4783．831的8个蛋白峰差异具有统计学意义；在发生与未发生转移食管癌患者血清中，发现3470．098、3395．789两个蛋白峰差异具有统计学意义。结论血清蛋白指纹图谱分析在食管癌的疗效和预后判断等方面显示出非常好的前景。

Objective To analyse serum proteomic patterns＂ differences in esophageal carcinoma patients by proteinchip technology and study its clinical value. Methods Surface-enhanced laser desorption / ionization proteomie patterns and WCX-2 protein chip were respectively used to detect the serum proteomic patterns in esophageal carcinoma patients according to before and after radiation, IMRT or 2-dimensional radiotherapy and metastasis or not. The data were analyzed by using Biomarker Wizard software. Results 8 differential protein peaks whose M/Z were 2863, 3884.213, 1699.600, 1371.126, 8454.341, 1141.881, 1473.377, 2086.908 and 2785.016 were significant before and after radiotherapy. 8 protein peaks whose M/Z were 3470.098, 2011.529, 3395.789, 2639.055, 1511.632, 11488.520, 9546.555 and 4783.831 were significant between IMRT and 2-dimensional radiotherapy. 2 protein peaks whose M/Z were 3470.098, 3395.789 were significant between metastasis and not metastasis. Conclusion Serum surface-enhanced laser desorption / ionization proteomie patterns analysis has good prospects in evaluating therapeutic effect and prognosis of esophageal carcinoma patients.