激素预处置对兔ARDS血清细胞因子的影响

Effects of Glucocorticoids Pretreatment on Serum CytOkine Levels of Rabbits with ARDS

目的:观察激素(地塞米松)预处置对兔油酸型急性呼吸窘迫综合症(ARDS)血清TNF-a、IL-8和IL-10的影响,探讨激素治疗ARDS的理论基础。方法:经兔耳缘静脉注射油酸复制兔ARDS模型,用酶联免疫吸附试验测定血清中TNF-a、IL-8和IL-10水平,结合血气分析检测及肺组织病理学改变进行观察分析。结果:ARDS组兔血清TNF-a、IL-8水平明显高于对照组(P<0.01);IL-10水平明显低于对照组(P<0.01)。地塞米松预处置组血清IL-10水平有上升恢复,高于ARDS组(P<0.01)。地塞米松预处置组血清TNF-a、IL-8水平降低,低于ARDS组(p<0.01)。动脉血气分析及肺组织病理学有相应改变。结论:TNF-a、IL-8和IL-10在兔油酸型ARDS炎症中可能起重要作用,地塞米松可能通过抑制TNF-a、IL-8过度分泌,促进IL-10合成而减轻肺损伤。

Objective： To observe the effects mechanism of glueocorticoids （dexamethasone） pretreatment on II, -6, IL-8, TNF-a, IL-IO levels in serum of rabbits with oleic acid ARDS. Methods： rabbits＇ ARDS model was repli- cated through injecting oleic acid to rabbits＇ veins under ears and the levels of IL-8, TNF-a, IL-10 in serum was measured using enzyme linked immunoserbent assay （ELISA）. Results： In ARDS group the levels of IL-8, TNF- a in serum were significantly higher than that of the control group（P〈O. O1 ）. wheras the levels of IL-IO in serum was no- ticeably lower than that of the control group （P（O. 01） .In dexamethasone pretreatment group the levels of IL-8, TNF-g in serum were significantly lower than that of the ARDS group （P 〈 0. 01 ） whereas the levels of IL- 10 in serum was significantly higher than that of the ARDS group （P〈0. 01 ）. Conclusions： IL-8, TNF-a, IL-IO may play important roles in the inflammatory process of oleic acid ARDS in rabbits while the dexamethasone may relieve lung injuries by inhibiting excessive discharging of IL-8 and TNF-a.