无氮饲粮法与饥饿法对测定扬州鹅内源性氨基酸排泄量的影响

Effects of Nitrogen-Free Diet Method and Fasting Method on Endogenous Amino Acid Losses of Yangzhou Geese

本试验旨在研究无氮饲粮法及饥饿法测定去盲肠鹅及正常鹅内源性氨基酸排泄量的差异。选用成年扬州鹅公鹅12只,随机分成2组,每组6只,对其中一组进行去盲肠手术,采用24 h禁食+24 h全收粪法进行试验。结果表明:无氮饲粮法测定的内源性氨基酸排泄量中,正常组的丝氨酸、亮氨酸、苯丙氨酸显著高于去盲肠组(P<0.05),去盲肠组的精氨酸极显著高于正常组(P<0.01),其余氨基酸差异均不显著(P>0.05)。饥饿法测定的内源性氨基酸排泄量中,正常组的天冬氨酸与缬氨酸显著高于去盲肠组(P<0.05),其余氨基酸差异均不显著(P>0.05)。由此表明,无氮饲粮法与饥饿法测定扬州鹅内源性氨基酸排泄量并不完全相同,大部分氨基酸之间存在极显著差异,饥饿法测定的多数内源性氨基酸排泄量低于无氮饲粮法。

Endogenous amino acid losses in intact and caecectomized geese were determined by nitrogen-free diet method （NFD） and fasting method （FAS） in this study and the differences of the two methods were com- pared. Twelve adult male Yangzhou geese were randomly divided into two groups with six birds in each group, and one group was chosen to be caecetomized. The geese were fasted for 24 h and the endogenous amino acid losses were detected by collecting all excrement in 24 h. The results showed that endogenous serine, leucine, and phenylalanine losses in caecectomized group determined by NFD were significantly lower than those in in- tact group （P 〈 0.05 ）, endogenous arginie loss in caecectomized group was extremely significantly higher than that in intact group （ P 〈 0.01 ）, and the other endogenous amino acid losses determined by NFD were similar （ P 〉 0.05 ）. The endogenous aspartate and valine losses in intact group determined by FAS were sig- nificantly higher than those in caecectomized group （ P 〈 0.05 ）, and the other endogenous amino acid losses determined by FAS were similar （ P 〉 0.05 ）. It is concluded that endogenous amino acid losses in intact and caecectomized geese determined by NFD and FAS are different, most of endogenous amino acid losses are ex- tremely significantly different, and all most endogenous amino acid losses determined by NDF are higher than those determined by FAS.