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Yes相关蛋白在直肠癌中的表达及意义 被引量：4

Expression of Yes-associated protein in rectal cancer
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摘要 目的研究Yes相关蛋白（YAP）在直肠癌中表达水平的变化，探讨其在直肠癌中的意义。方法用反转录聚合酶链反应（RT-PCR）、Western bloting方法检测30例直肠癌患者和10例正常体检者直肠黏膜组织中YAPmRNA及蛋白的表达情况。结果直肠癌黏膜组织中YAPmRNA的表达水平显著高于正常直肠黏膜组织（73．2±11．1比10．4±4．1，P〈0．05）；直肠癌黏膜组织中YAP蛋白的表达水平显著高于正常直肠黏膜组织（65．7±9．5比9．2±4．3，P〈0．05）。结论YAP的高表达与直肠癌的发生有关。 Objective To study the change of Yes-associated protein （YAP） expression in rectal cancer and to explore the significance of YAP in rectal cancer. Methods Reverse transcriptase polymerase chain reaction （I^F-PCR） and Western bloting technology were used to detect mRNA and protein expression of YAP in 30 patients with rectal cancer and 10 cases of normal rectal mucosa tissue. Results The expressions of mRNA and protein of YAP in rectal cancer were significantly higher than those in normal rectal mucosa tissue （73.2 ±11.1 vs. 10.4 ±4.1, 65.7 ±9.5 vs. 9.2 ±4.3,P〈0.05）. Conclusion YAP high expression is related to the occurrence of rectal cancer.
作者 陈志山 杨昌毅 洪黎清 林再全
机构地区 福建省南安市医院普外科
出处 《中国医师进修杂志》 2013年第5期11-13,共3页 Chinese Journal of Postgraduates of Medicine
基金 福建省泉州市科技计划（2010275）
关键词 直肠肿瘤 逆转录聚合酶链反应 Yes相关蛋白 WESTERN bloting技术 Rectal neoplasms Reverse transcriptase polymerase chain reaction Yes-associated protein Western bloting technology
分类号 R735.37 [医药卫生—肿瘤]
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参考文献8

  • 1Edgar BA. From cell structure to transcription: Hippo forges a new path. Cell, 2006,124(2) : 267-273.
  • 2Harvey KF, Pfleger CM, Hariharan IK. The Drosophila Mst ortholog, hippo, restricts growth and cell proliferation and promotes apoptosis. Cell, 2003,114(4) :457-467.
  • 3Zhao B,Ye X, Yu J, et al. TEAD mediates YAP-dependent gene induction and growth control. Genes Dev, 2008,22 (14):1962- 1971.
  • 4Zhou D, Conrad C, Xia F, et al. Mstl and Mst2 maintain hepatocyte quiescence and suppress hepatocellular carcinoma development through inactivation of the Yapl oncogene. Cancer Cell, 2009,16(5 ) :425-438.
  • 5Wang Y, Dong Q, Zhang Q, et al. Overexpression of yes-associated protein contributes to progression and poor prognosis of non-small- cell lung cancer. Cancer Sci, 2010,101 (5) : 1279-1255.
  • 6Orr B, Bai H, Anders R, et al. Yes-associated protein 1 is widely expressed in human brain tumors and promotes glioblastoma growth. J Neuropathol Exp Neurol, 2011,70(7 ) : 568-577.
  • 7Xu MZ, Yao TJ, Lee NP, et al. Yes-associated protein is an independent prognostic marker in hepatocellular carcinoma. Cancer, 2009,115 ( 19 ) : 4576-4585.
  • 8陈志山,洪黎清,杨昌毅,林再全.Yes相关蛋白在结直肠癌组织中的表达及其临床意义[J].世界华人消化杂志,2011,19(27):2890-2894. 被引量：3

二级参考文献16

  • 1侯彦强,娄加陶,彭亮,周琳,倪健,孔宪涛,仲人前.结直肠癌组织中ECM1基因水平的测定及临床意义[J].世界华人消化杂志,2007,15(17):1960-1964. 被引量：7
  • 2Overholtzer M, Zhang J, Smolen GA, Muir B, Li W, Sgroi DC, Deng CX, Brugge JS, Haber DA. Trans- forming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon. Proc Natl Acad Sci U S A 2006; 103:12405-12410.
  • 3Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J, Li L, Zheng P, Ye K, Chin- naiyan A, Halder G, Lai ZC, Guan KL. Inactiva- tion of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. Genes Dev 2007; 21:2747-2761.
  • 4Lam-Himlin DM, Daniels JA, Gayyed MF, Dong J, Maitra A, Pan D, Montgomery EA, Anders RA. The hippo pathway in human upper gastroin- testinal dysplasia and carcinoma: a novel onco- genic pathway. Int J Gastrointest Cancer 2006; 37: 103-109.
  • 5SongJM KeltonG WangJL.结直肠癌常用筛查方法综沭[J].中围今科医学,2008,:1115-1120.
  • 6Brunner T, Mueller C. Apoptosis in disease: about shortage and excess. Essays Biochem 2003; 39:119-130.
  • 7Steinhardt AA, Gayyed MF, Klein AP, Dong J, Maitra A, Pan D, Montgomery EA, Anders RA. Ex- pression of Yes-associated protein in common solid tumors. Hum Patho12008; 39:1582-1589.
  • 8Dong J, Feldmann G, Huang J, Wu S, Zhang N, Comerford SA, Gayyed MF, Anders RA, Maitra A, Pan D. Elucidation of a universal size-control mechanism in Drosophila and mammals. Cell 2007; 130:1120-1133.
  • 9Pan D. Hippo signaling in organ size control Genes Dev 2007; 21:886-897.
  • 10Sudol M. Yes-associated protein (YAP65) is a pro- line-rich phosphoprotein that binds to the SH3 do- main of the Yes proto-oncogene product. Oncogene1994; 9:2145-2152.

共引文献2

同被引文献33

  • 1Riley LB, Desai DC. The molecular basis of cancer and the development of targeted therapy[J]. Snrg Clin North Am 2009, 89 ( 9 ) : 1-15.
  • 2Wang Y,Dong Q, Zhang Q, et al.Overexpression of yes- associated protein contributes to progression and poor prognosis of non-small-cell lung cancer[J]. Cancer Sci, 2010, 101 (21) :1279- 1285.
  • 3Sudol M. Yes-associated protein ( YAP65 ) is a proline- rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product. Oncogene,1994, 9(8): 2145-2152.
  • 4Xu MZ, Yao TJ, Lee NP,et al. Yes-associated protein is an independent prognostic marker in hepatocellular carcinoma [J]. Cancer, 2009, 115(19): 4576-85.
  • 5D' Hoore A,Wolthuis A M. Laparoscopic low anterior resection and transanal pull - through for low rectal cancer., a Natural Orifice Specimen Extraction (NOSE) technique[J]. Colorectal Disease, 2011,13 ( s7 ) ; 28- 31.
  • 6Wolthuis A M, Cini C, Penninckx F, et al. Transanal single port access to facilitate distal rectal mobilization in laparoscopic rectal sleeve resection with hand-sewn coloanal anastomosis[J]. Techniques in coloproctolo gy,2012,16(2) :161-165.
  • 7Gong H ,Yu Y, Yao Y. Clinical value of preventativei- leostomy following ultra low anterior rectal resection [J]. Cell biochemistry and biophysics, 2013, 65 (3): 491-493.
  • 8Hiranyakas A, Ho YH. Laparoscopic ultralow anterior resection versus laparoscopic pull-through with co- loanal anastomosis for rectal cancers: a comparative study[J].The American Journal of Surgery, 2011,202(3):291-297.
  • 9Aytac E, Lavery IC, Kalady MF, et al. Impact of obesi- ty on operation performed, complications, and long- term outcomes in terms of restoration of intestinal con- tinuity for patients with mid and low rectal cancer[J]. Diseases of the Colon ~ Rectum, 2013,56 (6):689- 697.
  • 10Levitt MA,Dickie B,Pena A. Evaluation and treatment of the patient with Hirschsprung disease who is not doing well after a pull through proeedure[C]//Semi- nars in pediatric surgery. WB Saunders, 2010,19 ( 2 ) : 146-153.

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中国医师进修杂志
2013年 第5期

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