AP-PCR和set1、set2两基因PCR用于志贺菌基因多态性研究

Study on the polymorphism of shigella flexneri 2a by using AP-PCR and set1,set2 double-PCR

目的与方法 : 应用随机引物 PCR(简称 AP- PCR或 PAPD)和 set1、set2两基因 PCR方法分析了 71株F2 a志贺菌的基因多态性。 结果 :两种引物的 AP- PCR中 ,引物 12将 71株 F2 a志贺菌分为两种不同的基因型 ,引物 17和 set1、set2两基因 PCR将所有菌株分为四种不同的基因型 ,两种方法结合则可分为七种不同的基因型 ;其中 6 5株 F2 a志贺菌基因型相同 ,其余 6株各为独立的型别。本研究表明 AP- PCR和 set1、set2两基因 PCR为志贺菌基因多态性分析的有效手段 ,二者结合则更为完善 ;研究结果还表明 ,在我国南北不同地区不同时间分离到的无论是暴发株还是散发株 ,均具有相同的优势克隆。 结论 :上述菌株是引起国内菌痢散发和暴发的主要基因型 。

Objectives and Methods: APPCR and setl,set2 doublePCR were carried out in 71 strains of Shigella flexinert 2a,43 outbreak strains isolated from Inner Mongolia,28 strains isolated from Jiangmen. Results:All strains were divided into 2 different clones by using APPCR(perimer 12),four different clones by using APPCR(perimer 17) and setl,set2 doublePCR.Combining two methods,all strains were divided into 7 different clones,65 of 71 strains belong to a single clone,the others were not the same among themselves. Conclusions: APPCR and setl,set2 doublePCR were effective to analysis gene polymophism of Shigella.Majoring of 71strains,no matter isolated from different place and at different time,outbreak strains or sporadis,belong to a single clone,which caused Shigellosis in different places of our country at different times,must be given priority to research and control.