银杏叶提取物对普通小球藻Hg^(2+)毒害保护作用的研究

EGb's Protective Role on Hg^(2+) Toxicity of Chlorella Vulgaris

本文研究了Hg2 + 对普通小球藻的致毒效应与银杏叶提取物的保护作用。试验显示 :普通小球藻的生理代谢活动对Hg2 + 敏感 ,单Hg2 + 处理系列组 ,随着Hg2 + 浓度提高 ,普通小球藻生长量降低 ;可溶性蛋白含量、SOD活性、总抗氧化能力等在Hg2 + 0～ 3mg L- 1范围内应激升高 ,4～ 5mg L- 1时升高幅度下降 ;而O2 - 、MDA、脂质氢过氧化物含量均在供处理Hg2 + 浓度范围内持续上升 ,表现出Hg2 + 促进O2 - 、MDA与脂质氢过氧化物的积累 ,显示出Hg2 + 的毒害效应。Hg2 + 加银杏叶提取物 (EGb)复合处理普通小球藻结果表明 :EGb可以显著提高普通小球藻的生物量 ;促进普通小球藻的生长 ;减少普通小球藻内O2 -的生成 ,减缓普通小球藻内SOD活性与总抗氧化能力的提高 ,最终降低MDA与脂质氢过氧化物的积累 ,从而显著减轻Hg2 + 的毒害作用。

The paper makes a study on the effects of Hg 2+ on Chlorella Vulgaris in mimic Hg 2+ polluted environment and Egb's protective role.The results show that the metabolism of Chlorella Vulgaris is sensitive to Hg 2+ With the increase of the Hg 2+ concentration,its growth rate is lowered.Soluble proten content,SOD activity and total antioxidant capacity increase when Hg 2+ concentration is under 3mgL -1 . From 4 to 5 mgL -1 ,the range of increase is down,while O 2 -,MDA and lipid hydroperoxide contents increase continuously.These indicate that Hg 2+ promotes the accumulation of O 2 -,MDA and lipid hydroperoxide and is poisonous to Chlorella Vulgaris.The result of Hg 2+ plus EGb treatment shows that EGb can improve Chloreklla Vulgaris growth rate,decrease the O 2 - output,retard the increase of O 2 -, SOD activity and antioxidant capacity and finally reduce the accumulation of MDA and lipid hydroperoxide so as to mitigate the poison of Hg 2+ 。